妊娠期正己烷暴露对雄性仔代大鼠生殖发育影响

发布时间：2018-09-07 10:23

【摘要】：目的探讨妊娠期正己烷暴露对雄性仔代大鼠生殖发育的影响。方法 24只Wistar孕鼠随机分为4组,于孕期第1~20 d静式吸入0(对照组)、360、1 800、9 000 mg/m3正己烷;雄性仔鼠从出生后第9 d(PND9)开始称重,PND69处死,计算睾丸及附睾脏器系数;组织切片观察睾丸病理学改变;计算精子活率、活力、密度及畸形率;测定血清睾酮水平和睾丸丙二醛含量。结果正己烷9 000 mg/m3组雄性仔鼠体重增长明显低于对照组,睾丸脏器系数[(1.05±0.07)mg/g]低于对照组[(1.12±0.05)mg/g](P0.05),精子活率和活力低于对照组;雄性仔鼠血清睾酮含量[(1.22±0.45)ng/m L]低于对照组[(2.53±0.99)ng/m L](P0.05);正己烷9 000 mg/m3组雄性仔鼠睾丸组织出现曲精小管排列疏松、精原细胞萎缩、曲精小管管壁变薄等病理改变;各组雄性仔鼠睾丸丙二醛含量无明显差异。结论妊娠期高剂量正己烷暴露可通过非脂质过氧化途径介导明显抑制雄性仔代生殖发育。
[Abstract]:Objective to investigate the effect of n-hexane exposure during pregnancy on reproductive development of male offspring rats. Methods Twenty-four pregnant Wistar rats were randomly divided into 4 groups. The pregnant rats were inhaled 0 (control group) on the 1st day of gestation (control group) by 360,800 mg/m3 / 9 000 mg/m3 n-hexane, and the male rats were killed by weighing PND69 from the 9th day after birth (PND9), and the visceral coefficients of testis and epididymis were calculated. Pathological changes of testis were observed by histopathology, sperm motility, density and malondialdehyde (malondialdehyde) were measured. Results in n-hexane 9 000 mg/m3 group, the body weight and testis organ coefficient [(1. 05 卤0. 07) mg/g] were significantly lower than those in the control group [(1. 12 卤0. 05) mg/g] (P0.05). The sperm motility and motility were lower than those in the control group. The content of serum testosterone in male rats [(1.22 卤0.45) ng/m / L] was lower than that in control group [(2.53 卤0.99) ng/m / L] (P0.05), and the testicular tissue of male rats in n-hexane 9 000 mg/m3 group had loose arrangement of seminiferous tubules, atrophy of spermatogonia and thinning of tubules. There was no significant difference in malondialdehyde (MDA) content in testis of male rats in each group. Conclusion High dose n-hexane exposure during pregnancy can significantly inhibit the reproductive development of male offspring through non-lipid peroxidation pathway.
【作者单位】：福建卫生职业技术学院;福建医科大学公共卫生学院;
【基金】：国家自然科学基金(30972514) 福建省医学创新项目(2014-CXB-9)
【分类号】：R114

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