3%苦参碱水剂急性毒性及致突变作用研究

发布时间：2018-09-08 09:06

【摘要】：目的：检测3%苦参碱水剂急性经口、经皮LD50、急性吸入LC50、对眼、皮肤刺激或腐蚀作用大小、皮肤变态反应性及其致突变作用。方法：根据《农药登记毒理学试验方法》(GB15670-1995)进行急性经口、急性经皮、急性吸入毒性、急性皮肤刺激强度、眼刺激性和皮肤变态反应(致敏)试验；应用鼠伤寒沙门氏菌回复突变试验(Ames试验),小鼠骨髓多染红细胞微核试验,小鼠淋巴瘤细胞株(L5178Y)TK基因突变试验,人外周血淋巴细胞染色体畸变试验进行3%苦参碱水剂的致突变作用研究。结果：3%苦参碱水剂对雌、雄性大鼠经口LD50均为4640mg/kg；经皮LD50均2000mg/kg；雌、雄大鼠吸入LC50均2000mg/m3；皮肤刺激强度平均分值为0.25；平均眼刺激积分指数(I.A.O.I)为6,眼刺激平均指数(M.I.O.I)48h后5；动物皮肤致敏率为0%。Ames试验中,3%苦参碱水剂各剂量组回变菌落数均未超过溶剂对照组回变菌落数的2倍,实验结果为阴性；与相应对照组比较,3%苦参碱水剂对小鼠骨髓多染红细胞微核率无增加作用,对小鼠淋巴瘤细胞株(L5178Y)TK基因无致突变效应,对人外周血淋巴细胞染色体无畸变作用,其差异均无统计学意义(P0.05)。结论：在本实验条件下,3%苦参碱水剂急性经口、急性经皮、急性吸入均属低毒；对皮肤无刺激性,对眼睛有轻度刺激性；对皮肤无致敏作用,属低毒农药。3%苦参碱水剂无明显致突变作用。
[Abstract]:Aim: to detect the effects of acute oral administration of 3% matrine water on eyes, skin irritation or corrosion, skin hypersensitivity and its mutagenicity by percutaneous LD50, inhalation of LC50,. Methods: acute oral, acute percutaneous, acute inhalation toxicity, acute skin irritation, eye irritation and skin allergy (sensitization) tests were performed according to the method of pesticide registration toxicology (GB15670-1995). The reverse mutation test of Salmonella typhimurium (Ames), micronucleus test of bone marrow polychromatic erythrocytes and TK gene mutation test of mouse lymphoma cell line (L5178Y) were used. The mutagenicity of 3% matrine was studied by chromosome aberration test of human peripheral blood lymphocytes. Results the average oral LD50 of female and male rats was 4640 mg / kg; percutaneous LD50 was 2000mg / kg; female and male rats inhaled LC50 2000mg / m3; the average score of skin stimulation was 0.25; the mean eye stimulation score (I.A.O.I) was 6; the mean eye stimulation index (M.I.O.I) was 5 hours after 48 hours. The sensitizing rate of animal skin was less than 2 times of that of the solvent control group, and the results were negative. Compared with the control group, 3% matrine water had no effect on micronucleus rate of bone marrow polychromatic erythrocytes, no mutagenic effect on TK gene of mouse lymphoma cell line (L5178Y) and no aberration on chromosome of human peripheral blood lymphocytes. The difference was not statistically significant (P0.05). Conclusion: under this experimental condition, 3% matrine hydrochloric acid is low toxic, has no irritation to the skin, has slight irritation to the eyes, and has no sensitizing effect on the skin, acute percutaneous inhalation and acute inhalation are all of low toxicity, no irritation to the skin, no irritation to the eyes, no sensitization to the skin, It is a low toxic pesticide. 3% matrine has no obvious mutagenicity.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114

【相似文献】