亚慢性镧暴露对大鼠海马氨基酸类神经递质表达影响

发布时间：2018-09-12 21:24

【摘要】：目的探讨镧对大鼠神经行为影响及海马组织氨基酸类神经递质释放的改变。方法健康成年雌性Wistar大鼠24只,随机分为对照组及低、中、高剂量染镧组(0.25%、0.50%、1.00%La Cl3),仔鼠自母鼠怀孕至断乳后1个月饮用不同剂量氯化镧;穿梭箱实验检测仔鼠神经行为改变,高效液相色谱法检测仔鼠海马中兴奋性氨基酸递质及抑制性氨基酸递质含量。结果与对照组比较,中、高剂量染镧组仔鼠接受电击时间、主动逃避潜伏期时间[分别为(26.00±8.11)、(36.38±5.88)与(6.50±2.45)、(9.38±3.50)s]]均延长,差异有统计学意义(P0.05),表现出学习记忆损伤;与对照组比较,中、高剂量染镧组仔鼠海马兴奋性氨基酸递质谷氨酸(Glu)、天冬氨酸(Asp)含量[分别为(8.16±2.16)、(12.05±4.86)与(8.91±3.48)、(12.33±4.57)μmol/gpro]升高,中、高剂量染镧组仔鼠海马抑制性氨基酸递质甘氨酸(Gly)、γ-氨基丁酸(GABA)、牛磺酸(Tau)含量[分别为(9.95±3.41)、(13.25±5.03)、(10.93±3.53)、(14.50±4.90)与(10.10±3.18)、(13.78±5.28)μmol/gpro]升高,差异有统计学意义(P0.05);呈剂量效应关系。结论亚慢性镧染毒可导致仔鼠学习记忆能力下降,其机制可能与仔鼠海马组织内兴奋性及抑制性氨基酸神经递质含量升高有关。
[Abstract]:Objective to investigate the effect of lanthanum on neurobehavioral behavior and the release of amino acid neurotransmitters from hippocampus in rats. Methods Twenty-four healthy adult female Wistar rats were randomly divided into two groups: control group and low dose group. High dose Lanthanum group (0.25% 0.50 + 1.00 La Cl3) was used to drink different doses of lanthanum chloride from pregnancy to one month after weaning. The contents of excitatory amino acid transmitters and inhibitory amino acid transmitters in hippocampus of newborn rats were determined by high performance liquid chromatography. Results compared with the control group, the time of electric shock and active escape latency of rats in the high dose lanthanum group [(26.00 卤8.11), (, 36.38 卤5.88) vs (6.50 卤2.45), (, 9.38 卤3.50) s] were prolonged, the difference was significant (P0.05). The contents of glutamate (Glu), (Asp) in hippocampus of rats exposed to lanthanum at high dose [(8.16 卤2.16), (12.05 卤4.86) and (8.91 卤3.48), (12.33 卤4.57) 渭 mol/gpro] were increased, respectively. The contents of glycine (Gly), 纬 -aminobutyric acid (GABA), taurine (Tau) in hippocampus [(9.95 卤3.41), () 13.25 卤5.03), (10.93 卤3.53), (14.50 卤4.90) and (10.10 卤3.18), (13.78 卤5.28) 渭 mol/gpro] increased in high dose group (P0.05). Conclusion Subchronic lanthanum exposure can decrease the ability of learning and memory in rats, and its mechanism may be related to the increase of excitatory and inhibitory amino acid neurotransmitters in hippocampus.
【作者单位】：辽东学院医学院分子医学研究室;
【基金】：辽宁省博士启动基金(201501134) 辽东学院博士启动基金(2014003);辽东学院校级基金(2015JC006) 国家级大学生创新创业计划项目(201511779003)
【分类号】：R114

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