酵母联合高果糖饲料对大鼠嘌呤代谢影响

发布时间：2018-09-15 05:17

【摘要】：目的酵母联合高果糖饮食对大鼠嘌呤代谢影响及作用机制研究。方法选用雄性Wistar大鼠40只,随机分为四组,每组10只,分别为空白对照组、酵母对照组、酵母氧嗪酸钾组(高尿酸血症传统模型组)、酵母果糖组。分别在第2、4、6、8 w末剪尾取血,测定各组的血清尿酸(SUA)、血清尿素氮(BUN)、血清肌酐(SCr)水平。在第8w周末时,收集24h尿液,测定尿中尿酸(UUA)、肌酐(UCr),计算尿酸清除率(CUA)和肌酐清除率(CCR),测定血清腺苷脱氨酶(ADA)和黄嘌呤氧化酶(XOD)活性,并进行肾脏组织学病理切片观察。采用Western blotting检测大鼠肾脏有机阴离子转运子1(OAT1)、肾脏尿酸盐转运体(RST)、葡萄糖转运蛋白9(GLUT9)的蛋白表达水平。结果实验第2、4、6、8 w末,酵母果糖组大鼠血清SUA水平分别达到345.9、403.7,447.2和451.4μmol/L。与空白对照组相比,酵母果糖组SUA水平分别升高了66.5%、33.8%、37.5%和69.8%;而酵母氧嗪酸钾组(传统模型组)大鼠SUA水平也显著升高,显示造模成功。在第8 w,酵母果糖组与酵母氧嗪酸钾组大鼠SUA水平分别是空白对照组2.24倍和2.17倍,显示果糖具有氧嗪酸钾类似的促进尿酸生成作用。与嘌呤代谢相关的XOD和ADA活性分析显示,酵母果糖组大鼠血清和肝匀浆中XOD活性明显高于空白对照组(均P0.05);而ADA活力也显著高于空白对照组(均P0.05)。与空白对照组相比,酵母果糖组大鼠24h尿量明显增加,CUA明显降低(均P0.05)。组织学分析显示,在实验第8 w,酵母果糖组大鼠肾小管管腔间质偶见结晶物沉积,无明显纤维化,肾小管、肾小球组织结构无明显异常。Western blotting结果显示,与空白对照组相比,酵母果糖组大鼠肾脏OAT1水平明显下降,RST水平明显升高(均P0.05)。结论果糖可增强酵母诱发高尿酸血症的作用,对肾的副作用较小,可作为开展嘌呤代谢研究的良好模型;但摄入高嘌呤食物时应避免果糖过量摄入。
[Abstract]:Objective to study the effect and mechanism of yeast combined with high fructose diet on purine metabolism in rats. Methods Forty male Wistar rats were randomly divided into four groups: control group (n = 10), potassium yeast oxalate group (traditional hyperuricemia model group) and yeast fructose group (n = 10). Blood samples were taken from the tail at the end of 8 weeks at the end of the 6th week of the 2nd week. Serum uric acid (SUA), urea nitrogen (BUN), serum creatinine (SCr) were measured in each group. At the end of the 8th week, urine samples were collected for 24 hours. Uric acid clearance rate (CUA) and creatinine clearance rate (CCR),) in urine were determined by (UUA), creatinine (UCr), and creatinine clearance (CCR), respectively. The activities of serum adenosine deaminase (ADA) and xanthine oxidase (XOD) were measured. The expression of organic anion transporter 1 (OAT1) and renal uric acid transporter (RST), glucose transporter 9 (GLUT9) was detected by Western blotting. Results at the end of 8 weeks, the serum SUA levels in the yeast fructose group were 345.9403.7447.2 and 451.4 渭 mol/L., respectively. Compared with the blank control group, the SUA level in the yeast fructose group increased by 66.5% and 69.8%, respectively, while the SUA level in the traditional model group (traditional model group) also increased significantly, indicating that the model was successful. At the 8th week, the levels of SUA in the yeast fructose group and the yeast oxazinate potassium group were 2.24 and 2.17 times higher than those in the blank control group, respectively, indicating that fructose had a similar effect on uric acid production. The analysis of XOD and ADA activity related to purine metabolism showed that the activity of XOD in serum and liver homogenate of rats in yeast fructose group was significantly higher than that in blank control group (P0.05), and the activity of ADA was significantly higher than that of blank control group (P0.05). Compared with the blank control group, the 24h urine volume of rats in the yeast fructose group was significantly increased and CUA significantly decreased (P0.05). Histological analysis showed that in the 8th week of the experiment, the tubulointerstitial crystals were occasionally deposited in the yeast fructose group, and there was no obvious fibrosis, and there was no obvious abnormality in the structure of the renal tubules and glomeruli. Western blotting showed that, compared with the blank control group, the rat renal tubulointerstitial structure in the yeast fructose group was similar to that in the control group. The level of renal OAT1 in yeast fructose group decreased significantly (P0.05). Conclusion fructose can enhance the effect of yeast inducing hyperuricemia, and the side effect on kidney is less, so fructose can be used as a good model to study purine metabolism, but fructose overdose should be avoided when consuming high purine food.
【作者单位】：青岛大学公共卫生学院营养与食品卫生学教研室;
【基金】：国家自然科学基金(No.81373000)
【分类号】：R151

【相似文献】