饮用水有机提取物对正常人肝细胞凋亡及炎性因子的影响

发布时间：2018-10-11 09:17

【摘要】：目的观察L02细胞经饮用水有机提取物染毒后所诱发的白介素6(interleukin 6,IL-6)和丙氨酸氨基转移酶(alanine transaminase,ALT)及细胞凋亡的改变。方法采用固相萃取法提取饮用水中有机污染物。将取处于对数生长期的正常人肝(L02)细胞分别暴露于分别暴露于含终浓度0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/ml有机提取物的培养液中暴露24、48、72 h,同时设溶剂对照(含0.1%DMSO培养液)和空白对照(培养液)。检测细胞凋亡率及细胞培养液上清中IL-6的含量和ALT的活力。结果与溶剂对照组比较,各剂量饮用水有机提取物染毒不同时间L02细胞的凋亡率均较高,除0.312 5、0.625 0 L/ml染毒24 h和0.312 5 L/ml染毒48 h组外,差异均有统计学意义(P0.05);各剂量饮用水有机提取物染毒不同时间L02细胞培养液上清中IL-6的含量均较高,差异均有统计学意义(P0.05);各剂量饮用水有机提取物染毒不同时间L02细胞培养液上清中ALT的活力均较高,除0.312 5、0.625 0 L/ml染毒24 h组外,差异均有统计学意义(P0.05)。且随着饮用水有机提取物染毒剂量的升高和染毒时间的延长,L02细胞的凋亡率及细胞培养液上清中IL-6含量和ALT活力均呈上升趋势。结论在本实验条件下,饮用水有机污染物可剂量依赖性及时间依赖性地诱导L02细胞炎性因子IL-6的分泌,引发炎症反应,诱导细胞凋亡,从而导致肝细胞损伤。
[Abstract]:Objective to observe the changes of interleukin 6 (interleukin 6), alanine aminotransferase (alanine transaminase,ALT) and apoptosis induced by organic extracts from drinking water in L02 cells. Methods solid phase extraction was used to extract organic pollutants from drinking water. The normal human liver (L02) cells at logarithmic growth stage were exposed to the medium containing the final concentration of 0.312 ~ 0.625 ~ 1.2500 ~ 2.500 ~ 5.000 L/ml for 24 ~ 4872 h, respectively. The cells were exposed to a solvent control (including 0.1%DMSO medium) and a blank control (culture medium) respectively. Apoptosis rate, IL-6 content and ALT activity in supernatant of cell culture medium were measured. Results compared with the solvent control group, the apoptosis rate of L02 cells was higher at different time after exposure to organic extracts from drinking water at different time points, except for the groups exposed for 24 h and 0.312 5 L/ml, respectively. The content of IL-6 in the supernatant of L02 cells was higher at different time after exposure to organic extracts from drinking water (P0.05). The activity of ALT in the supernatant of L02 cells exposed to different doses of drinking water organic extract at different time was higher than that of the control group (P 0.05), except for the treatment group of 0.312 ~ 0.625 L/ml for 24 h (P0.05). The apoptosis rate, IL-6 content and ALT activity in the supernatant of L02 cells were increased with the increase of drinking water organic extract dose and the prolongation of exposure time. Conclusion in this experiment, drinking water organic pollutants can induce IL-6 secretion of L02 cells in a dose-and time-dependent manner, induce inflammatory reaction, induce apoptosis and lead to hepatocyte damage.
【作者单位】：贵州医科大学公共卫生学院;
【基金】：贵州省科技厅基金(黔科合LH字[2014]7102) 贵阳市科学技术计划项目(筑科合同[2013103]19号)
【分类号】：R114

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