鹅骨胶原蛋白及肽对大鼠骨髓间充质干细胞增殖及向成骨分化的影响

发布时间：2018-10-12 20:10

【摘要】：本实验通过研究鹅骨胶原蛋白、含钙胶原蛋白、胶原肽、含钙胶原肽对大鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)增殖及向成骨细胞(Osteoblast,OB)分化的影响,阐述鹅骨胶原蛋白及多肽对骨质疏松的作用机制。采用MTT(噻唑蓝)法测定鹅骨胶原蛋白及多肽对BMSCs增殖的影响;碱性磷酸酶(Alkaline phosphatase,ALP)染色、ALP活性测定、茜素红染色和骨钙素(BGP)分泌量测定分析BMSCs成骨分化能力。结果表明,相较于对照组,试验组可促进BMSCs的增殖,第14d ALP染色试验组ALP染色阳性率显著高于对照组(p0.05),第7 d和第14 d鹅骨胶原蛋白及肽可显著提高ALP的活性表达(p0.05),第21 d茜素红染色试验组钙结节个数相较于对照组显著增加(p0.05),骨钙素分泌量试验组显著高于对照组(p0.05)。由此表明,大分子胶原蛋白、含钙大分子胶原蛋白、小分子胶原多肽、含钙小分子胶原多肽均可促进BMSCs的增殖及向成骨细胞分化,以含钙小分子胶原多肽作用效果最佳。
[Abstract]:In this study, the effects of goose bone collagen, calcium collagen, collagen peptide and calcium collagen peptide on the proliferation and differentiation of rat bone marrow mesenchymal stem cells (Bone marrow mesenchymal stem cells,BMSCs) into osteoblasts (Osteoblast,OB) were studied. The mechanism of action of goose bone collagen and polypeptide on osteoporosis was described. The effects of goose bone collagen and polypeptide on the proliferation of BMSCs were determined by MTT, alkaline phosphatase (Alkaline phosphatase,ALP) staining, ALP activity, alizarin red staining and osteocalcin (BGP) secretion assay were used to analyze the osteogenic differentiation ability of BMSCs. The results showed that compared with the control group, the experimental group could promote the proliferation of BMSCs. On the 14th day, the positive rate of ALP staining in the ALP staining group was significantly higher than that in the control group (p0.05). On the 7th and 14th days, the expression of ALP was significantly increased in the goose bone collagen and peptide (p0.05). The number of calcium nodules in the alizarin red staining group was higher than that in the control group on the 21st day. The osteocalcin secretion in the experimental group was significantly higher than that in the control group (p0.05). The results showed that macromolecular collagen, calcium containing macromolecular collagen, small molecular collagen polypeptide and calcium containing collagen peptide could promote the proliferation and differentiation of BMSCs into osteoblasts, and the calcium containing collagen peptide had the best effect.
【作者单位】：浙江省动物蛋白精深加工技术重点实验室宁波大学;南京师范大学食品科学与营养系;
【基金】：浙江省自然科学基金(LQ14C200002) 国家农业科技成果转化资金(2013GB2C200191) 宁波市科技局创新基金(2013C910017)
【分类号】：R151;TS201.4

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