牡蛎污染耐药性细菌调查及整合子检测
发布时间：2018-10-17 16:26
【摘要】：目的对水产品牡蛎污染耐药性细菌进行调查,并检测其携带整合子类型,为细菌耐药机制研究提供依据。方法利用抗性培养基平板筛选牡蛎标本中的耐药性细菌,用K-B法进行药敏试验,PCR法扩增Ⅰ型、Ⅱ型和Ⅲ型整合酶基因。结果 34份牡蛎标本分离出76株耐药株,其中超过90%的分离菌对四环素、氨苄西林耐药,诺氟沙星、左氧氟沙星、复方新诺明、环丙沙星、头孢唑啉和氯霉素耐药50%,有2株对耐亚胺培南细菌。整合酶基因检测结果显示80.26%耐药细菌携带Ⅰ型整合子,其中6株同时携带Ⅱ型整合子,未检测到Ⅲ型整合子。所有携带整合子的耐药细菌是多重耐药,不携带整合子耐药细菌中多重耐药占60%。结论牡蛎污染细菌的耐药性严重,且多重耐药与携带整合子有关,携带整合子细菌更易产生多重耐药,提示整合子可作为食品分离菌的耐药性检测靶标。
[Abstract]:Objective To investigate the drug-resistant bacteria of oyster contaminated with aquatic products and to detect the type of drug-resistant bacteria and provide the basis for the study of the mechanism of bacterial resistance. Methods The resistant bacteria in oyster samples were screened by using resistant culture medium plate. The susceptibility test was carried out by K-B method. PCR method was used to amplify type 鈪，
本文编号：2277252
[Abstract]:Objective To investigate the drug-resistant bacteria of oyster contaminated with aquatic products and to detect the type of drug-resistant bacteria and provide the basis for the study of the mechanism of bacterial resistance. Methods The resistant bacteria in oyster samples were screened by using resistant culture medium plate. The susceptibility test was carried out by K-B method. PCR method was used to amplify type 鈪，
本文编号：2277252
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