利用大鼠体外多器官细胞共培养方法检测红霉素、链霉素、多柔比星和白消安的细胞毒性

发布时间：2018-10-23 17:51

【摘要】：目的探讨采用大鼠体外多器官细胞共培养(Id MOC)方法检测药物靶细胞毒性的可能性。方法应用大鼠多器官原代细胞共培养模型,观察注射用乳糖酸红霉素浓度0.143,0.286,0.572,1.144,2.289和4.578 mmol·L-1,硫酸链霉素0.214,0.429,0.858,1.715,3.431和6.862 mmol·L-1,多柔比星4.310,8.620,17.242,34.483和68.967μmol·L-1,白消安0.508,1.015,2.03,4.06和8.12mmol·L-1,均作用24 h后,对心肌细胞、星形胶质细胞、肝细胞、肾皮质细胞和肺泡巨噬细胞形态的变化,并用MTT比色法测定并计算各IC50值。结果红霉素的毒性为:肺泡巨噬细胞星形胶质细胞心肌细胞肾皮质细胞肝细胞;链霉素的毒性为:肺泡巨噬细胞肾皮质细胞心肌细胞星形胶质细胞肝细胞;多柔比星的毒性为:星形胶质细胞肺泡巨噬细胞肾皮质细胞肝细胞心肌细胞;白消安的毒性为:星形胶质细胞心肌细胞肺泡巨噬细胞肾皮质细胞肝细胞。结论利用大鼠Id MOC模型可检出受试物多数已知的靶器官毒性,同时发现了一些可能的潜在毒性。
[Abstract]:Objective to investigate the possibility of multiorgan cell coculture (Id MOC) in detecting the cytotoxicity of drug target cells in vitro. Methods the co-culture model of rat primary cells with multiple organs was used. The concentrations of erythromycin lactate (0.143X) 0.286U 0.2874U 2.289 and 4.578 mmol L-1, streptomycin sulfate 0.214290.8580.8580.8580.8581.7153.431 and 6.862 mmol L-1, doxorubicin (4.3108.62020) 17.24234.483 and 68.967 渭 mol L-1were used to observe the effects on cardiomyocytes after 24 hours. The morphological changes of astrocytes, hepatocytes, renal cortex cells and alveolar macrophages were measured and calculated by MTT colorimetry. Results the toxicity of erythromycin was as follows: alveolar macrophages, astrocytes, renal cortical cells, hepatocytes, streptomycin, alveolar macrophages, renal cortical cells, cardiomyocytes, hepatocytes. The toxicity of doxorubicin was astrocytes, alveolar macrophages, renal cortical cells, hepatocytes, and oxacinol, astrocytes, alveolar macrophages, renal cortex cells, hepatocytes. Conclusion most of the known target organ toxicity can be detected by using rat Id MOC model, and some possible potential toxicity can be found at the same time.
【作者单位】：第二军医大学卫生毒理学教研室;杭州师范大学医学实验中心;湖南师范大学医学院预防医学系;
【基金】：湖南省科技计划项目资助课题(2012SK3128)~~
【分类号】：R114

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