生活饮用水中损伤性非苛养菌的耐药性分析
发布时间：2018-10-24 15:29
【摘要】：目的研究生活饮用水中损伤性非苛养菌的耐药性。方法采用增菌培养基和选择性培养基对某小区生活饮用水进行损伤菌分离,获得分离株后,利用16S rRNA基因测序法进行种属鉴定;采用Kirby-Bauer纸片扩散法进行细菌的抗菌药物敏感性试验。结果在分离的58株损伤性非苛养菌中,耐药菌占51.72%,包括肠球菌属、肠杆菌属、葡萄球菌属、不动杆菌属、假单胞菌属。其中80%的损伤菌表现出对两种及以上抗菌药物耐药。耐药菌对复方新诺明的耐药最为普遍,耐药率为63.33%;对磺胺甲VA唑的耐药次之,占43.33%。结论测试小区生活饮用水中的损伤性非苛养菌耐药性较为普遍,应加强关注其潜在的人群健康风险。
[Abstract]:Objective to study the drug resistance of damaged non-culture bacteria in drinking water. Methods Bacterization medium and selective medium were used to isolate the damaged bacteria from drinking water in a small area. The isolated strains were identified by 16s rRNA gene sequencing method. The antimicrobial susceptibility of bacteria was tested by Kirby-Bauer disk diffusion method. Results among the 58 strains of non-caustic injurious bacteria, drug-resistant bacteria accounted for 51.72%, including Enterococcus, Enterobacter, Staphylococcus, Acinetobacter, Pseudomonas. 80% of them showed resistance to two or more antimicrobial agents. The resistance of drug-resistant bacteria to compound sulfamethoxazole was the most common, the resistance rate was 63.33, and the resistance to sulfamethoxazole was the second, accounting for 43.33%. Conclusion the drug resistance of damaged non-culture bacteria in drinking water in residential areas is more common, and attention should be paid to the potential population health risks.
【作者单位】： 军事医学科学院卫生学环境医学研究所;
【基金】：国家自然科学基金面上项目(81372947)
【分类号】：R123.1
本文编号：2291773
[Abstract]:Objective to study the drug resistance of damaged non-culture bacteria in drinking water. Methods Bacterization medium and selective medium were used to isolate the damaged bacteria from drinking water in a small area. The isolated strains were identified by 16s rRNA gene sequencing method. The antimicrobial susceptibility of bacteria was tested by Kirby-Bauer disk diffusion method. Results among the 58 strains of non-caustic injurious bacteria, drug-resistant bacteria accounted for 51.72%, including Enterococcus, Enterobacter, Staphylococcus, Acinetobacter, Pseudomonas. 80% of them showed resistance to two or more antimicrobial agents. The resistance of drug-resistant bacteria to compound sulfamethoxazole was the most common, the resistance rate was 63.33, and the resistance to sulfamethoxazole was the second, accounting for 43.33%. Conclusion the drug resistance of damaged non-culture bacteria in drinking water in residential areas is more common, and attention should be paid to the potential population health risks.
【作者单位】： 军事医学科学院卫生学环境医学研究所;
【基金】：国家自然科学基金面上项目(81372947)
【分类号】：R123.1
【相似文献】
相关硕士学位论文 前2条
1 徐欢;城市污水处理系统中耐药性沙门氏菌及其耐药基因的分布研究[D];西安建筑科技大学;2014年
2 吴菡;建筑供水系统抗生素耐药性细菌的分布与灭活研究[D];北京交通大学;2016年
，本文编号：2291773
本文链接：https://www.wllwen.com/yixuelunwen/yufangyixuelunwen/2291773.html
