EGCG和维生素E对孕前期铅暴露仔鼠学习记忆能力的影响

发布时间：2018-10-25 15:14

【摘要】：目的:探讨表没食子儿茶素没食子酸酯(EGCG)和维生素E(Vit E)对铅暴露仔鼠学习记忆能力的影响。方法:将健康SPF级昆明雌鼠随机分为对照组(4只)和染毒组(20只)。染毒组饮2 g/L醋酸铅溶液染毒21 d,对照组饮无铅去离子水21 d。染毒结束后按雌雄比2∶1合笼饲养。分娩后第21天,将染毒组所产仔鼠随机分为7组,即生理盐水组、玉米油组、EGCG干预组(3.0 mg/kg)、VitE干预组(30 IU/kg)、联合干预1组(EGCG 3.0 mg/kg+VitE 30 IU/kg)、联合干预2组(EGCG 1.5 mg/kg+VitE 60 IU/kg)、联合干预3组(EGCG 6.0 mg/kg+VitE 15IU/kg),每组10只;随机选取10只对照组雌鼠所产仔鼠作为正常对照组。正常对照组仔鼠饮无铅去离子水,其余7组仔鼠灌胃相应试剂,共干预28 d。灌胃结束后,用Morris水迷宫测定小鼠学习记忆能力,用极谱仪测定小鼠血铅含量,用氧化酶试剂盒测定海马组织中SOD活性和MDA含量。结果:与正常对照组相比,生理盐水组和玉米油组逃避潜伏期延长,穿越平台次数减少,血铅含量升高,海马组织中SOD活性降低,MDA含量增加(P0.05)。与生理盐水组和玉米油组相比,各干预组逃避潜伏期缩短,穿越平台次数增多,血铅含量不同程度降低,联合干预1、3组SOD活性升高,MDA含量降低(P0.05)。结论:EGCG和VitE可能通过提高海马组织中SOD活性,改善仔鼠的学习记忆能力,对铅暴露仔鼠神经损伤有一定的保护作用。
[Abstract]:Aim: to investigate the effects of epigallocatechin gallate (EGCG) and vitamin E (Vit E) on learning and memory in lead exposed rats. Methods: healthy SPF female mice were randomly divided into control group (n = 4) and exposure group (n = 20). The exposure group drank 2 g / L lead acetate solution for 21 days, while the control group drank lead-free deionized water for 21 days. After exposure to the end of the male and female according to 2:1 cage feeding. On the 21st day after delivery, the rats in the exposed group were randomly divided into 7 groups, that is, the saline group. Corn oil group, EGCG intervention group (30 IU/kg), combined intervention group 1 (EGCG 3.0 mg/kg VitE 30 IU/kg), combined intervention group 2 (EGCG 1.5 mg/kg VitE 60 IU/kg), combined intervention group (EGCG 6.0 mg/kg VitE 15IU/kg), 10 rats in each group; Ten female rats of control group were randomly selected as normal control group. The rats in the control group drank lead-free deionized water, and the other 7 rats were treated with corresponding reagents for 28 days. The learning and memory ability of mice was measured by Morris water maze, the blood lead content was measured by polarography, the activity of SOD and the content of MDA in hippocampal tissue were measured by oxidase kit. Results: compared with the normal control group, the escape latency of normal saline group and corn oil group was prolonged, the times of crossing the plateau decreased, the blood lead content increased, the activity of SOD and the content of MDA in hippocampus decreased (P0.05). Compared with normal saline group and corn oil group, the escape latency of each intervention group was shorter, the number of crossing platform increased, the blood lead content decreased in varying degrees, the activity of SOD increased and the content of MDA decreased in the combined intervention group 1: 3 (P0.05). Conclusion: EGCG and VitE may enhance the activity of SOD in hippocampus and improve the ability of learning and memory of newborn rats, which may have a protective effect on the nerve injury of rats exposed to lead.
【作者单位】：郑州大学公共卫生学院营养与食品卫生学教研室;新乡医学院基础医学院;
【基金】：国家自然科学基金资助项目81172716
【分类号】：R114

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