洋葱多肽及蛋白对T2DM小鼠降血糖及降血脂作用研究

发布时间：2018-10-29 17:22

【摘要】：目的:研究洋葱多肽及蛋白的体内降糖降脂活性。方法:将造模成功的小鼠进行糖耐量实验和药效实验,利用各生化指标参数和动物组织切片,探究洋葱多肽及蛋白的体内降糖及降脂作用。结果:200 mg/kg bw洋葱蛋白及洋葱多肽的降糖效果达到盐酸二甲双胍降糖效果的70%及80%以上;洋葱多肽组则在60、120 min时的血糖值及糖耐量AUC均显著低于模型组,分别达到阳性对照组的87.34%、93.53%和92.44%;洋葱蛋白组的AST和UREA高于正常值但低于模型组,洋葱多肽组与阳性对照组各项指标均在阈值内。结论:洋葱蛋白和洋葱多肽能有效降低2型糖尿病小鼠血糖和血脂,有效提高其口服耐糖量,并能对受损的小鼠肝、肾予以一定程度的恢复,且洋葱多肽的作用优于洋葱蛋白。
[Abstract]:Objective: to study the hypoglycemic and lipid-lowering activity of onion peptides and proteins in vivo. Methods: the glucose tolerance test and pharmacodynamics test were carried out in the successful mice, and the effects of onion peptides and proteins on reducing blood sugar and lipid were studied by using biochemical parameters and animal tissue sections. Results: the hypoglycemic effect of 200 mg/kg bw onion protein and onion polypeptide reached 70% and more than 80% of that of metformin hydrochloride. The glucose level and glucose tolerance AUC of onion polypeptide group at 60120 min were significantly lower than that of model group, reaching 93.53% and 92.44% of the positive control group, respectively. The AST and UREA of onion protein group were higher than the normal value but lower than that of the model group. The indexes of onion polypeptide group and positive control group were all within the threshold value. Conclusion: onion protein and onion polypeptide can effectively reduce blood glucose and blood lipids in type 2 diabetic mice, increase their oral glucose tolerance, and recover the damaged liver and kidney to a certain extent, and the effect of onion polypeptide is better than onion protein.
【作者单位】： 武昌理工学院生命科学学院生物多肽糖尿病药物湖北省协同创新中心湖北省生物多肽糖尿病药物工程技术研究中心;
【基金】：国家级大学生创新训练项目(201612310004) 湖北省级大学生创新创业训练计划项目(201512310017) 湖北省高等学校优秀中青年科技创新团队计划项目(T201632) 武昌理工学院校级重点科研项目(2016K04) 武昌理工学院校级科研项目(2015X05)
【分类号】：R151
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本文编号：2298328