邻苯二甲酸-单-乙基己基酯对原代培养新生大鼠下丘脑神经元活性的影响及其氧化损伤作用

发布时间：2018-10-30 20:06

【摘要】：目的探讨邻苯二甲酸-单-乙基己基酯[mono-(2-ethylhexyl)phthalate,MEHP]对原代培养新生大鼠下丘脑神经元活性的影响及其氧化损伤作用。方法选取24 h内新生清洁级SD大鼠,取下丘脑神经元进行原代培养,分别加入含终浓度0(溶剂对照)、10~(-12)、10~(-9)、10~(-6)、10~(-3)、1.0 mmol/L MEHP的培养液暴露24 h。采用MTT法测定下丘脑神经元的活性,试剂盒测定超氧化物歧化酶(SOD)的活力和丙二醛(MDA)的含量。结果与溶剂对照组比较,仅1.0 mmol/L MEHP暴露组新生大鼠下丘脑神经元的存活率较低,差异有统计学意义(P0.05);且随着MEHP暴露剂量的升高,新生大鼠下丘脑神经元的存活率呈下降趋势。与溶剂对照组比较,仅1.0 mmol/L MEHP暴露组新生大鼠下丘脑神经元上清液中的MDA含量升高,差异有统计学意义(P0.05);而各MEHP暴露组新生大鼠下丘脑神经元上清液中的SOD活力均无明显改变。结论MEHP可抑制原代培养新生大鼠下丘脑神经元活性,并具有氧化损伤作用,提示其可能是MEHP神经毒性机制之一。
[Abstract]:Objective to investigate the effect of mono- (2-ethylhexyl) phthalate,MEHP on the activity of hypothalamic neurons and its oxidative damage in neonatal rats. Methods Neonatal clean grade SD rats were selected for primary culture of hypothalamic neurons within 24 hours, and 10 ~ (-12), 10 ~ (-9), 10 ~ (-6), 10 ~ (-3), 10 ~ (-6), 10 ~ (-6), 10 ~ (-6), 10 ~ (-6), 10 ~ (-6), 10 ~ (-6) and 10 ~ (-3), respectively. 1. 0 mmol/L MEHP culture medium was exposed for 24 h. The activity of hypothalamic neurons was measured by MTT assay, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured by kit. Results compared with the solvent control group, the survival rate of hypothalamic neurons in the 1.0 mmol/L MEHP exposure group was lower than that in the control group (P0.05). The survival rate of hypothalamic neurons decreased with the increase of MEHP exposure dose. Compared with the solvent control group, the content of MDA in the supernatant of hypothalamic neurons in the 1.0 mmol/L MEHP exposure group was significantly higher than that in the control group (P0.05). However, the activity of SOD in the supernatant of hypothalamic neurons in all MEHP exposure groups did not change significantly. Conclusion MEHP can inhibit the activity of hypothalamic neurons in primary cultured neonatal rats and has the effect of oxidative injury, suggesting that MEHP may be one of the neurotoxic mechanisms of MEHP.
【作者单位】：天津医科大学公共卫生学院;
【基金】：国家自然科学基金(81273027)
【分类号】：R114

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