砷冶炼厂工人砷甲基化代谢与肝和皮肤损伤及LncRNAs表达的关系

发布时间：2018-10-31 09:49

【摘要】：[目的]探讨职业性砷接触工人砷甲基化代谢转化模式与肝和皮肤损伤、长链非编码RNA(LncRNAs)表达的关系。[方法]2013年10月选择云南省文山州两个砷冶炼厂的112名工人作为接触组,无砷接触经历的居民41人为对照组。对接触组进行健康监护体检,检查肝和皮肤损伤情况并分为4组(无损伤组、肝损伤组、皮肤损伤组、肝和皮肤均损伤组);用带有砷预处理系统的原子吸收分光光度计检测尿中无机砷、甲基砷酸和二甲基砷酸含量,并计算一、二级甲基化指数;用实时荧光定量PCR法检测外周血3种与恶性肿瘤发生关系相关的LncRNAs(MEG3、TUG1和HOTAIR)表达。[结果]职业性砷接触工人年龄(33.9±16.8)岁,工龄(16.6±9.7)月,其中无损伤组42人,肝损伤组29人,皮肤损伤组21人,肝和皮肤均损伤组20人。各接触组尿中3种砷化合物浓度均高于对照组(均P0.05),一、二级甲基化指数低于对照组(均P0.05);肝损伤组一级甲基化指数和皮肤损伤组一、二级甲基化指数低于肝和皮肤均损伤组(P0.05)。与对照组相比,无损伤、皮肤损伤、肝和皮肤均损伤组外周血MEG3和HOTAIR表达升高,无损伤、肝和皮肤均损伤组工人TUG1表达升高(P0.05)。与肝和皮肤均损伤组比较,无损伤和肝损伤组MEG3表达降低,肝损伤组TUG1和HOTAIR表达降低(P0.05)。[结论]职业砷接触工人不同砷甲基化代谢模式与肝和皮肤损伤、外周血LncRNAs表达之间存在关联。
[Abstract]:[objective] to investigate the relationship between the metabolic transformation of arsenic methylation and liver and skin injury and the expression of long chain noncoding RNA (LncRNAs) in workers exposed to occupational arsenic. [methods] in October 2013, 112 workers from two arsenic smelters in Wenshan Prefecture, Yunnan Province were selected as exposure group and 41 residents without arsenic exposure as control group. The patients in the contact group were examined for liver and skin injury and were divided into 4 groups (no injury group, liver injury group, skin injury group, liver and skin injury group). The content of inorganic arsenic, methyl arsenic acid and dimethyl arsenic acid in urine was determined by atomic absorption spectrophotometer with arsenic pretreatment system, and the first and second order methylation index was calculated. The expression of LncRNAs (MEG3,TUG1 and HOTAIR) in peripheral blood was detected by real-time fluorescence quantitative PCR. [results] the age of the workers exposed to occupational arsenic was (33.9 卤16.8) years old and the working age was (16.6 卤9.7) months. Among them, 42 were in the no-injury group, 29 in the liver injury group, 21 in the skin injury group, and 20 in both the liver and skin injury group. The concentrations of three arsenic compounds in urine of each exposure group were higher than those of the control group (P0.05), and the second order methylation index was lower than that of the control group (P0.05). The first methylation index and the second methylation index of liver injury group and skin injury group were lower than that of liver and skin injury group (P0.05). Compared with the control group, the expression of MEG3 and HOTAIR in peripheral blood was increased in the groups of no injury, skin injury, liver and skin injury, and the expression of TUG1 was increased in the group of liver and skin injury (P0.05). Compared with the liver and skin injury group, the expression of MEG3 was decreased in the non-injury group and the liver injury group, and the expression of TUG1 and HOTAIR was decreased in the liver injury group (P0.05). [conclusion] there is a correlation between the metabolic patterns of arsenic methylation and liver and skin injury, and the expression of LncRNAs in peripheral blood of workers exposed to arsenic.
【作者单位】：云南省疾病预防控制中心慢性非传染病防制所;
【基金】：国家自然科学基金(编号:81160343,30860238)
【分类号】：R135

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