慢性砷染毒对大鼠睾丸肝细胞生长因子及其受体c-Met表达的影响

发布时间：2018-11-04 17:33

【摘要】：目的探讨砷对大鼠睾丸中肝细胞生长因子(hepatocyte growth factor,HGF)及其受体c-Met表达的影响。方法将40只健康清洁雄性SD大鼠随机分为4组,分别为对照(蒸馏水)组和2.4、12.0、60.0 mg/L亚砷酸钠染毒组,每组10只。采用自由饮用方式进行染毒,连续染毒6个月。测定大鼠睾丸组织内HGF及其受体c-Met的阳性表达及精子的凋亡情况。结果与对照组比较,各浓度亚砷酸钠染毒组大鼠睾丸组织内HGF、c-Met的阳性表达水平均较低,差异均有统计学意义(P0.05);且随着亚砷酸钠染毒浓度的升高,大鼠的HGF、c-Met的阳性表达水平呈下降趋势。与对照组比较,各浓度亚砷酸钠染毒组大鼠的精子凋亡率均升高,差异有统计学意义(P0.05);且随着亚砷酸钠染毒浓度的升高,大鼠的精子凋亡率呈上升趋势。结论慢性砷染毒可能通过抑制HGF-c-Met通路引起大鼠睾丸组织的损伤及精子凋亡。
[Abstract]:Objective to investigate the effect of arsenic on the expression of hepatocyte growth factor (hepatocyte growth factor,HGF) and its receptor c-Met in rat testis. Methods Forty healthy and clean male SD rats were randomly divided into 4 groups: control group (distilled water) and sodium arsenite group (2.40.12.0 mg/L), 10 rats in each group. The free drinking method was used for 6 months. The positive expression of HGF and its receptor c-Met and the apoptosis of spermatozoa were measured in rat testis. Results compared with the control group, the positive expression level of HGF,c-Met in testis of rats exposed to sodium arsenite was lower than that of control group (P0.05). With the increase of sodium arsenite concentration, the positive expression of HGF,c-Met in rats decreased. Compared with the control group, the rate of sperm apoptosis of rats exposed to sodium arsenite was significantly higher than that of the control group (P0.05), and with the increase of the concentration of sodium arsenite, the rate of sperm apoptosis showed an increasing trend. Conclusion chronic arsenic exposure may induce testicular injury and sperm apoptosis in rats by inhibiting HGF-c-Met pathway.
【作者单位】：贵州医科大学基础医学院;岳阳市岳阳楼区人民医院内科;遵义医药高等专科学校;
【基金】：贵州省科学技术基金(黔科合人才团队[2014]4005号;贵州省科学技术基金(黔科合LH字[2015]7568号) 遵义市科技计划(遵市科合社字[2014]01号)
【分类号】：R114

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