米胚芽对成年小鼠糖耐量影响

发布时间：2018-11-06 14:08

【摘要】：目的探讨米胚芽对成年小鼠糖耐量的影响及作用机制。方法 6周龄ICR小鼠30只,按体重随机分为对照组、低、高剂量米胚芽组,分别给予基础饲料、添加10%和40%米胚芽饲料饲养4周,实验结束后测空腹血糖、胰岛素,计算胰岛素敏感指数(ISI),进行口服葡萄糖耐量试验(OGTT);取小鼠血清和肝组织测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛含量;采用荧光实时定量PCR(RT-PCR)测定小鼠肝脏组织中葡萄糖激酶(GCK)和脂肪组织中葡萄糖转运体-4(GLUT-4)mRNA表达水平。结果与对照组比较,高剂量米胚芽组小鼠ISI(-2.59±0.33)明显升高、0.5 h血糖[(6.75±2.20)mmol/L]与血糖曲线下面积(12.81±3.19)下降(P0.05);与对照组比较,高剂量米胚芽组小鼠肝脏组织中GSH-Px、SOD酶活力[分别为(44.34±11.96)、(34.88±7.70)U/gprot]升高,丙二醛含量[(1.57±0.43)nmol/m L]降低;与对照组比较,低、高剂量米胚芽组小鼠血清中SOD活力[分别为(48.45±2.54)、(53.27±2.51)U/m L]升高,MDA含量[分别为(1.44±0.25)、(1.46±0.35)nmol/m L]降低(P均0.05);与对照组比较,高剂量米胚芽组小鼠肝脏组织GCK mRNA表达水平上调,低高剂量米胚芽组小鼠脂肪组织GLUT-4 mRNA表达水平上调(P0.05)。结论米胚芽可提高成年小鼠的糖耐量水平,其机制可能与其抗氧化活性和调控糖代谢相关基因表达有关。
[Abstract]:Objective to investigate the effect and mechanism of rice germ on glucose tolerance in adult mice. Methods Thirty 6-week-old ICR mice were randomly divided into two groups according to their body weight: control group, low-dose group and high-dose group. They were fed with basic diet, supplemented with 10% and 40% rice germ for4 weeks, respectively. Fasting blood glucose and insulin were measured at the end of the experiment. Calculation of insulin sensitivity index (ISI),) for oral glucose tolerance test (OGTT);) The activity of superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) were measured in serum and liver of mice. The expression of glucokinase (GCK) in mouse liver and glucose transporter 4 (GLUT-4) mRNA in adipose tissue were measured by real-time fluorescence quantitative PCR (RT-PCR). Results compared with the control group, ISI in the high-dose rice germ group (-2.59 卤0.33) was significantly increased, and the blood glucose [(6.75 卤2.20) mmol/L] and the area under the blood glucose curve (12.81 卤3.19) were decreased (P0.05). Compared with the control group, the activity of GSH-Px,SOD enzyme in the liver of the high-dose rice germ group [(44.34 卤11.96), (34.88 卤7.70) U/gprot, respectively] increased and the content of malondialdehyde [(1.57 卤0.43) nmol/m L] decreased. Compared with the control group, the serum SOD activity [(48.45 卤2.54), (, 53.27 卤2.51) U / mL] and the content of MDA [(1.44 卤0.25)] in the low and high dose rice embryo group were increased, respectively. (1. 46 卤0. 35) nmol/m L] decreased (P 0. 05). Compared with the control group, the expression of GCK mRNA in liver was up-regulated in the high-dose rice embryo group and the adipose tissue GLUT-4 mRNA expression level in the low-dose rice embryo group (P0.05). Conclusion Rice germ can improve glucose tolerance in adult mice and its mechanism may be related to its antioxidant activity and regulation of gene expression related to glucose metabolism.
【作者单位】：福建医科大学公共卫生学院卫生检验与检疫学系环境与健康重点实验室;泉州鲤城泉珍农产品专业合作社;
【基金】：福建省科技厅星火计划项目(2015S0006)
【分类号】：R151

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