气相色谱法和离子色谱法测定饮料中甜蜜素的方法比较

发布时间：2018-11-16 12:32

【摘要】：目的对气相色谱法和离子色谱法测定饮料中甜蜜素的方法进行比较。方法气相色谱法测定甜蜜素按照国标方法 GB/T 5009.97-2003进行,并对其进行了改进;离子色谱法测定甜蜜素时,样品经水稀释过0.22μm滤膜后,经Cleanert-IC C18柱净化,Ion Pac AS 16离子柱分离,用KOH洗脱,电导检测器检测,外标法定量。结果两种方法的线性范围为12.5~250 mg/kg,气相色谱法测定甜蜜素的检出限为1.0 mg/kg,定量限为3.0 mg/kg,加标回收率在92.6%~106%之间,相对标准偏差小于5%;离子色谱法测定甜蜜素的检出限为3.0 mg/kg,定量限为10 mg/kg,加标回收率在91.8%~104%之间,相对标准偏差小于4%。经配对t检验处理,气相色谱法和离子色谱法对饮料中甜蜜素的测定结果无统计学差异(t=-1.719,P0.05)。结论离子色谱法的前处理简单,污染小;而气相色谱法的检出限、成本更低。
[Abstract]:Objective to compare the methods of determination of cyclamate in beverage by gas chromatography and ion chromatography. Methods the determination of cyclamate by gas chromatography was carried out in accordance with the national standard method GB/T 5009.97-2003 and improved. In the determination of cyclamate by ion chromatography, the sample was diluted by water over 0.22 渭 m filter membrane, then purified by Cleanert-IC C18 column, then separated by KOH elution, detected by conductance detector and quantified by external standard method. Results the linear range of the two methods was 12.5 渭 m 250 mg/kg, for the determination of cyclamate by gas chromatography. The detection limit was 1.0 mg/kg, the quantitative limit was 3.0 mg/kg, and the recovery rate was between 92.66% and 100.6%. The relative standard deviation is less than 5. The detection limit for determination of cyclamate by ion chromatography was 3.0 mg/kg, the quantitative limit was 10 mg/kg, the recovery rate was between 91.8% and 104%, and the relative standard deviation was less than 4%. After paired t test, there was no statistical difference between gas chromatography and ion chromatography on the determination of cyclamate in beverage (t = -1.719 P 0.05). Conclusion the pretreatment of ion chromatography is simple and the pollution is small, and the detection limit of gas chromatography is lower.
【作者单位】：北京市西城区疾病预防控制中心;
【分类号】：R155.5

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