双酚AF暴露对胚胎期和幼鱼期斑马鱼的毒性效应

发布时间：2018-11-18 20:08

【摘要】：为明确不同发育阶段的斑马鱼对BPAF(双酚AF)暴露的易感性,采用暴露试验法初步研究了BPAF对胚胎期和幼鱼期斑马鱼的发育毒性.结果表明:1BPAF暴露可延缓胚胎期斑马鱼的发育和孵化,使其出现心包水肿、卵黄囊异常、心率下降、心脏搏动停止等症状.2暴露96 h后,ρ(BPAF)为2.0、2.5、3.0 mg/L暴露组胚胎期斑马鱼的畸形率高达100%,暴露24 h致畸的EC50(半数效应浓度)为2.00 mg/L,暴露96 h的LC50(半数致死浓度)为1.84 mg/L.3暴露72 h后,对照组幼鱼期斑马鱼鱼鳔发育缺陷率为0;除ρ(BPAF)为1.0 mg/L暴露组外,其余BPAF暴露组幼鱼期斑马鱼鱼鳔发育缺陷率为100%.4随着暴露时间的延长以及中毒程度的加深,幼鱼期斑马鱼出现的中毒症状依次表现为心包水肿、卵黄囊水肿、背脊弯曲、心跳停止,暴露48和72 h时其心包水肿的EC50分别为1.76、1.56 mg/L,暴露96 h的LC50为1.77 mg/L.胚胎期和幼鱼期斑马鱼对BPAF暴露响应的差异分析显示,幼鱼期斑马鱼对BPAF暴露的反应更为敏感,幼鱼期斑马鱼的心包水肿症状可作为BPAF毒性响应的最佳指标之一.
[Abstract]:In order to determine the susceptibility of zebrafish at different developmental stages to BPAF (bisphenol A F) exposure, the developmental toxicity of BPAF to zebrafish at embryonic and juvenile stages was preliminarily studied by exposure test. The results showed that 1BPAF exposure could delay the development and hatching of zebrafish at embryonic stage, and induce pericardial edema, abnormal yolk sac, heart rate decrease and cardiac arrest. The deformity rate of embryonic zebrafish exposed to 蟻 (BPAF) was 2. 0 and 2. 5 mg/L. The malformation rate of zebrafish at embryonic stage was as high as 100. The EC50 (half effect concentration) of 24 h exposure was 2. 00 mg/L,. After exposure to LC50 (LC50) of 1.84 mg/L.3 for 72 h at 96 h, the developmental defect rate of juvenile zebrafish in control group was 0. In addition to 蟻 (BPAF) 1. 0 mg/L exposure group, the developmental defect rate of juvenile zebrafish in BPAF exposure group was 100. 4 with the prolongation of exposure time and the deepening of poisoning. The toxic symptoms of zebrafish in juvenile stage were pericardial edema, yolk sac edema, dorsal ridge curvature, cardiac arrest, and the EC50 of pericardial edema was 1.76 mg/L, at 48 h and 72 h after exposure, respectively. The LC50 of 96 h exposure was 1.77 mg/L.. The difference between embryonic and juvenile zebrafish responses to BPAF exposure showed that juvenile zebrafish were more sensitive to BPAF exposure. Pericardial edema of juvenile zebrafish could be regarded as one of the best indicators of BPAF toxicity response.
【作者单位】：海南大学环境与植物保护学院;海南大学海口市环境毒理学重点实验室;海南医学院热带医学与检验医学院;
【基金】：海南省中西部高校提升综合实力工作资金资助项目(ZXBJH-XK004)
【分类号】：R114

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