鱼油对PM2.5所致心血管炎性和氧化损伤拮抗作用

发布时间：2018-11-22 18:24

【摘要】：目的探讨深海鱼油对大气细颗粒物(PM2.5)引起的心血管系统中的炎性损伤和氧化应激损伤影响。方法将SD雄性大鼠随机分为鱼油低、中、高剂量组,鱼油对照组,溶剂对照组(玉米油)和PM2.5染毒组;鱼油组大鼠先进行深海鱼油灌胃28 d,再进行PM2.5气管滴注染毒隔天1次,共3次,PM2.5染毒期间同时进行鱼油灌胃,PM2.5染毒组先正常饲养28 d,再进行PM2.5染毒;取血清检测白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(hs-CRP)、丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)。结果与溶剂对照组比较,PM2.5染毒组大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分别为(24.94±2.06)、(821.45±14.26)ng/m L、(3.10±0.28)mg/L、(15.88±1.41)nmol/m L]升高,GSH、GSH-Px、TSOD活性[(4.62±0.37)μmol/L、(289.28±30.65)活力单位、(239.26±4.97)U/m L]降低(均P0.05);与PM2.5染毒组比较,鱼油高剂量组大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分别为(16.67±0.23)、(73.01±3.80)ng/m L、(1.36±0.22)mg/L、(11.82±0.79)nmol/m L]下降,GSH、GSH-Px、T-SOD活性[(24.05±3.83)μmol/L、(531.33±40.39)活力单位、(273.36±3.92)U/m L]升高,呈剂量效应关系(均P0.05)。结论深海鱼油对大气PM2.5所致心血管系统炎性反应和氧化应激损伤具有明显拮抗作用。
[Abstract]:Objective to investigate the effects of deep-sea fish oil on inflammatory injury and oxidative stress injury in cardiovascular system induced by atmospheric fine particulate matter (PM2.5). Methods male SD rats were randomly divided into low, medium and high dose groups, fish oil control group, solvent control group (corn oil) and PM2.5 exposure group. The rats in the fish oil group were fed with deep-sea fish oil for 28 days and then injected with PM2.5 once every other day for 3 times. The rats in the PM2.5 group were fed with fish oil at the same time during PM2.5 exposure. The rats in the PM2.5 group were fed normally for 28 days and then PM2.5. Serum levels of interleukin-6 (IL-6), tumor necrosis factor 伪 (TNF- 伪), hypersensitive C-reactive protein (hs-CRP) and malondialdehyde (MDA),) total superoxide dismutase (T-SOD) were measured. Glutathione (GSH) and glutathione peroxidase (GSH-Px). Results compared with solvent control group, the contents of IL-6,TNF- 伪 and hs-CRP,MDA in serum of rats exposed to PM2.5 were (24.94 卤2.06), (821.45 卤14.26) ng/m L, (3.10 卤0.28) mg/L, respectively. The activity of GSH,GSH-Px,TSOD [(4.62 卤0.37) 渭 mol/L, (289.28 卤30.65), (239.26 卤4.97) U / mL] was increased (P 0.05). Compared with PM2.5 exposure group, the contents of IL-6,TNF- 伪 and hs-CRP,MDA in serum of high dose group of fish oil were (16.67 卤0.23), (73.01 卤3.80) ng/m L, (1.36 卤0.22) mg/L, respectively. The activity of GSH,GSH-Px,T-SOD [(24.05 卤3.83) 渭 mol/L, (531.33 卤40.39), (273.36 卤3.92) U / mL] decreased, and the activity of GSH,GSH-Px,T-SOD increased. There was a dose-effect relationship (P0.05). Conclusion Deep-sea fish oil has obvious antagonistic effect on cardiovascular system inflammatory response and oxidative stress injury induced by atmospheric PM2.5.
【作者单位】：复旦大学公共卫生学院公共卫生安全教育部重点实验室;
【基金】：国家自然科学基金(81172617)
【分类号】：R151

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