X射线对人周围血淋巴细胞Gadd45 mRNA表达影响

发布时间：2018-11-25 11:14

【摘要】：目的分析不同受照剂量和不同受照时间对人周围血淋巴细胞生长抑制和DNA损伤基因45(Gadd45)mRNA相对表达水平的影响,探讨Gadd45基因作为生物剂量计的可能性。方法 1剂量-效应关系研究。以0.00、0.10、0.25、0.50、1.00、2.00、3.00和5.00 Gy剂量X射线离体照射健康人周围血样,分离淋巴细胞进行检测;2时间-效应关系研究。以2.00 Gy剂量X射线离体照射健康人周围血样,分离淋巴细胞后分别于培养0、3、6、12、24和48 h时收获细胞。采用实时荧光定量聚合酶链反应法检测Gadd45 mRNA相对表达水平。结果 1 0.00~0.50 Gy剂量范围内,Gadd45 mRNA相对表达水平随照射剂量的增加而增加,回归方程为y=1.056 9+7.132 2 x[决定系数(R2)=0.992,P0.01]。0.50 Gy剂量组Gadd45 mRNA相对表达水平达到峰值,为0.00 Gy剂量组的4.53倍;0.50~5.00 Gy剂量范围内,Gadd45 mRNA相对表达水平呈波动性下降。22.00 Gy剂量照射后3~12 h,Gadd45 mRNA相对表达水平随照射时间的增加而增加,回归方程为y=-6.366 0+4.965 0 x(R2=0.932,P0.05)。照射后12 h时间组Gadd45 mRNA相对表达水平达到峰值,为0 h时间组的57.68倍;Gadd45 mRNA相对表达水平在照射后24~48 h大幅度下降,照射后48 h时间组为0 h时间组的2.08倍。结论 X射线照射可致Gadd45 mRNA表达上调,在一定剂量和时间范围内具有良好的剂量-效应和时间-效应关系;但Gadd45基因作为新型生物剂量计仍需进一步研究验证。
[Abstract]:Objective to analyze the effects of different doses and time of irradiation on the growth inhibition of human peripheral blood lymphocytes and the relative expression of DNA damage gene 45 (Gadd45) mRNA, and to explore the possibility of Gadd45 gene as a biological dosimeter. Methods 1 the dose-effect relationship was studied. The peripheral blood samples of healthy people were irradiated in vitro with the doses of 0.000.10 ~ 0.25 ~ 0.50 ~ 1.00 ~ 1.00 ~ 2.00 ~ 3.00 and 5.00 Gy respectively, and the relationship between time and effect was studied. Peripheral blood samples of healthy people were irradiated with 2. 00 Gy dose of X ray in vitro. Lymphocytes were isolated and cultured for 24 h and 48 h, respectively. The relative expression of Gadd45 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction. Results within the dose range of 0.50 Gy, the relative expression of Gadd45 mRNA increased with the increase of irradiation dose. The regression equation was 1.056 9 7.132 2 x [determination coefficient (R2) = 0.992 2]. P0.01. The relative expression of Gadd45 mRNA in 0.50 Gy group reached the peak value, 4.53 times of that in 0.00 Gy group. Within the dose range of 0.50 Gy 5.00 Gy, the relative expression level of Gadd45 mRNA decreased, and the relative expression level of Gadd45 mRNA increased with the increase of irradiation time at 3h 12 h after 22.00 Gy dose irradiation. The regression equation was Yi-6.366 0 4.965 x (R _ 2N _ (0.932) P _ (0.05). The relative expression level of Gadd45 mRNA reached the peak at 12 h after irradiation, which was 57.68 times of that in 0 h group, and the relative expression level of Gadd45 mRNA decreased significantly at 24 h after irradiation 48 h after irradiation, and 2.08 times as much as 0 h at 48 h after irradiation. Conclusion the expression of Gadd45 mRNA can be upregulated by X-ray irradiation, which has a good dose-effect and time-effect relationship within a certain dose and time range, but the Gadd45 gene as a new biological dosimeter needs further study and verification.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;
【基金】：广东省职业病防治重点实验室(2012A061400007) 广东省职业病防治院课题(Z201004)
【分类号】：R144

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