柚子籽油营养分析及其微胶囊制备
发布时间：2018-11-26 10:53
【摘要】：柚子籽油是芸香科柑橘亚科柑橘属植物柚子(Citrus paradise Macf.)的种子油脂,尚未得到有效利用。本研究对柚子籽油进行了营养分析,研究表明它富含不饱和脂肪酸以及柠檬苦素等物质,具有较高的营养价值。由于柚子籽油本身具有不良苦味,而且所含营养物质不稳定,易氧化,为了掩盖柚子籽油的苦味并提高其稳定性,本研究通过饱和水溶液法制备β-CD/柚子籽油微胶囊,以期为柚子籽油的合理开发利用提供一定参考。主要研究内容及结果如下： 1.评价柚子籽油营养分析 柚子籽油的相对密度0.968g/mL,折光指数1.471,过氧化值5.63mmol/kg,酸值1.06mgKOH/g,皂化值196.46mg KOH/g,碘值97.896g/100g,各理化指标都比较适宜。GC分析表明柚子籽油中含有66.95%的不饱和脂肪酸,其中LA35.4%(其中CLA0.58%)、油酸26.38%、α-亚麻酸3.92%；含有33.02%的饱和脂肪酸,其中棕榈酸29.60%、硬脂酸2.86%。 FT-IR分析表明,柚子籽油主要有10个吸收峰,表现在：3750-3000cm-1区间的O-H伸缩振动,3000-2800cm-1区间的饱和C-H伸缩振动,1900-1630cm-1区间的羰基伸缩振动,1475-1300cm-1区间的饱和C-H面内弯曲振动,1000-650cm-1区间的不饱和C-H面外弯曲振动。由分析结果可推断柚子籽油含有不饱和脂肪酸,也可进一步为柚子籽油鉴别、营养分析提供信息。 2.HPLC法检测柚子籽油中柠檬苦素含量 建立了柚子籽油中柠檬苦素的HPLC检测方法,色谱条件：色谱柱为Agilent Zorbax Extend-C18(4.6mm×250mm,5μm),流动相为乙腈-水(45：55),流速0.8mL/min,检测波长215nm,柱温30℃。结果表明柠檬苦素在48～384μg/mL (R2=0.9997)浓度范围内线性关系良好,加样平均回收率为99.87%, RSD=1.33%(n=3)。在此条件下测得柚子籽油中柠檬苦素含量为327.61μg/g 3.β-CD／柚子籽油微胶囊的制备 以饱和水溶液法制备p-CD／柚子籽油微胶囊。研究表明投料比(p-CD/柚子籽油,g/mL)、包埋时间、包埋温度对微胶囊包埋率影响程度大小为：包埋温度包埋时间投料比。在单因素试验基础上通过响应面分析法优化制备工艺,确定最佳工艺为：投料比4.5,包埋时间132min,包埋温度47℃,在此工艺条件下包埋率达92.18%。4.p-CD/柚子籽油微胶囊质量评价 物相鉴定试验表明β-CD已经成功包埋柚子籽油形成微胶囊,且包埋过程中柚子籽油化学成分未发生变化,扫描电镜下可见微胶囊颗粒较圆整,表面附着有小颗粒。微胶囊平均粒径为1.58pm、含水率3.19%、密度0.452g/cm3、溶解度70.31%、休止角36°；微胶囊载油量为74.32%；热稳定性试验表明p-CD/柚子籽油微胶囊质量减少量低于柚子籽油；预测货架期可达到182d,高于柚子籽油(74d),因此,可以判断柚子籽油经包埋后对热稳定性提高。 综上所述,柚子籽油具有较高营养价值,且饱和水溶液法操作简便,所需设备要求不高,成本较低,制得的β-CD/柚子籽油微胶囊包埋率较高,热稳定性良好,具有一定推广开发价值。
[Abstract]:Pomelo seed oil is (Citrus paradise Macf.) The seed oil has not been used effectively. The nutritive analysis of pomelo seed oil showed that it was rich in unsaturated fatty acids and limonin and had high nutritional value. In order to conceal the bitter taste of pomelo seed oil and improve its stability, 尾-CD/ pomelo seed oil microcapsules were prepared by saturated aqueous solution method. In order to provide a certain reference for the reasonable development and utilization of pomelo seed oil. The main contents and results are as follows: 1. The relative density of pomelo seed oil was 0.968 g / mL, refractive index was 1.471, peroxide value was 5.63 mmol / kg, acid value was 1.06 mg / kg, saponification value of 196.46mg KOH/g, was 97.896 g / 100 g. GC analysis showed that grapefruit seed oil contained 66.95% unsaturated fatty acids, including LA35.4% (CLA0.58%), oleic acid 26.38, 伪 -linolenic acid 3.92; It contains 33.02% saturated fatty acids, including 29.60% palmitic acid and 2.86% stearic acid. FT-IR analysis showed that grapefruit seed oil had 10 absorption peaks, which were characterized by O-H stretching vibration in 3750-3000cm-1 region, saturated C-H stretching vibration in 3000-2800cm-1 region and carbonyl stretching vibration in 1900-1630cm-1 region. The bending vibration in saturated C-H plane of 1475-1300cm-1 interval and the out-of-plane bending vibration of unsaturated C-H region in 1000-650cm-1 interval. It can be concluded that pomelo seed oil contains unsaturated fatty acids, and it can also provide information for identification and nutrition analysis of pomelo seed oil. Determination of limonin in pomelo seed oil by 2.HPLC the HPLC method for the determination of limonin in pomelo seed oil was established. The chromatographic conditions were as follows: Agilent Zorbax Extend-C18 (4.6mm 脳 250mm-1 5 渭 m),) was used as the mobile phase of acetonitrile-water (45:55). The flow rate was 0.8 mL / min, the detection wavelength was 215 nm and the column temperature was 30 鈩，
本文编号：2358360
[Abstract]:Pomelo seed oil is (Citrus paradise Macf.) The seed oil has not been used effectively. The nutritive analysis of pomelo seed oil showed that it was rich in unsaturated fatty acids and limonin and had high nutritional value. In order to conceal the bitter taste of pomelo seed oil and improve its stability, 尾-CD/ pomelo seed oil microcapsules were prepared by saturated aqueous solution method. In order to provide a certain reference for the reasonable development and utilization of pomelo seed oil. The main contents and results are as follows: 1. The relative density of pomelo seed oil was 0.968 g / mL, refractive index was 1.471, peroxide value was 5.63 mmol / kg, acid value was 1.06 mg / kg, saponification value of 196.46mg KOH/g, was 97.896 g / 100 g. GC analysis showed that grapefruit seed oil contained 66.95% unsaturated fatty acids, including LA35.4% (CLA0.58%), oleic acid 26.38, 伪 -linolenic acid 3.92; It contains 33.02% saturated fatty acids, including 29.60% palmitic acid and 2.86% stearic acid. FT-IR analysis showed that grapefruit seed oil had 10 absorption peaks, which were characterized by O-H stretching vibration in 3750-3000cm-1 region, saturated C-H stretching vibration in 3000-2800cm-1 region and carbonyl stretching vibration in 1900-1630cm-1 region. The bending vibration in saturated C-H plane of 1475-1300cm-1 interval and the out-of-plane bending vibration of unsaturated C-H region in 1000-650cm-1 interval. It can be concluded that pomelo seed oil contains unsaturated fatty acids, and it can also provide information for identification and nutrition analysis of pomelo seed oil. Determination of limonin in pomelo seed oil by 2.HPLC the HPLC method for the determination of limonin in pomelo seed oil was established. The chromatographic conditions were as follows: Agilent Zorbax Extend-C18 (4.6mm 脳 250mm-1 5 渭 m),) was used as the mobile phase of acetonitrile-water (45:55). The flow rate was 0.8 mL / min, the detection wavelength was 215 nm and the column temperature was 30 鈩，
本文编号：2358360
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