蓝莓花色苷对实验性糖尿病小白鼠肝脏抗氧化功能的影响

发布时间：2018-11-27 14:21

【摘要】：目的:研究蓝莓花色苷对实验性糖尿病小鼠肝脏抗氧化能力和超微结构的影响。方法:建模成功的40只小鼠分为4组,模型对照组、模型低、中、高剂量组分别以0.24、0.48、0.96 mg/(g·d)的蓝莓花色苷剂量灌胃模型组小鼠,20只正常小鼠分为正常对照组和正常高剂量组,正常高剂量组参考模型高剂量组的花色苷用量,模型对照组和正常对照组用等体积蒸馏水替代花色苷溶液。4周后,测定小鼠空腹血糖含量,肝脏的丙二醛含量、谷胱甘肽过氧化物酶活性、总抗氧化能力、抑制O_2~-·和·OH能力,另外利用透射电子显微镜观察各组小鼠肝脏细胞超微结构的变化。结果:蓝莓花色苷可显著降低糖尿病小鼠的血糖含量(P0.05)和肝脏中的丙二醛含量(P0.05),小鼠肝脏抑制·OH、O_2~-·能力、谷胱甘肽过氧化物酶活性及总抗氧化能力均显著增加(P0.05),同时可以保持肝脏细胞的完整性。蓝莓花色苷高剂量组效果最好。结论:蓝莓花色苷具有一定增强肝脏组织的抗氧化能力和保护肝细胞的作用。
[Abstract]:Aim: to study the effects of blueberry anthocyanins on liver antioxidation and ultrastructure in experimental diabetic mice. Methods: forty mice were divided into 4 groups, model control group, low, middle and high dose group were treated by oral administration of blueberry anthocyanin at 0.240.48 ~ 0.96 mg/ (g d), respectively. Twenty normal mice were divided into two groups: normal control group and normal high dose group. The dosage of anthocyanin in reference model high dose group in normal high dose group and the same volume distilled water instead of anthocyanin solution in model control group and normal control group. The contents of fasting blood glucose, malondialdehyde (MDA), glutathione peroxidase (GSH) activity, total antioxidant capacity, and inhibition of O _ 2O _ 2 and OH were measured in mice. In addition, the ultrastructure of liver cells in each group was observed by transmission electron microscope. Results: blueberry anthocyanin significantly decreased the content of blood glucose (P0.05) and malondialdehyde (P0.05) in the liver of diabetic mice, and the ability of inhibiting OH,O_2~- in liver of mice. Glutathione peroxidase activity and total antioxidant capacity were significantly increased (P0.05), while maintaining the integrity of liver cells. The effect of blueberry anthocyanin was the best in high dose group. Conclusion: blueberry anthocyanins can enhance the antioxidation ability of liver tissue and protect hepatocytes.
【作者单位】：吉林农业大学园艺学院;大连民族大学生命科学学院;
【基金】：农业部2011公益性行业(农业)科研专项(201103037) 吉林省财政厅项目(2012004) 吉林省科技厅发展计划项目(20110828) 大连民族大学自主科研基金项目(DC201501082)
【分类号】：R151

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