低水平焦炉逸散物对接触人群尿中8-羟基脱氧鸟苷影响分析

发布时间：2018-11-27 19:43

【摘要】：目的探讨低水平焦炉逸散物(COE)接触与焦炉作业工人尿中8-羟基脱氧鸟苷(8-OHd G)的剂量-效应关系。方法采用横断面调查方法,以某焦化厂263名焦炉作业工人为接触组,以某氧气厂113名非焦炉作业工人为对照组。检测2组人群工作场所空气中COE短时间接触浓度(CSTEL)。分别采用高效液相色谱法和超高效液相色谱-质谱法测定研究对象尿中1-羟基芘(1-OHP)和8-OHd G水平。结果对照组和接触组工作场所空气中COE的CSTEL分别为0.020~0.070(0.042±0.018)、0.100~0.320(0.208±0.069)mg/m3;接触组人群工作场所空气中COE的CSTEL高于对照组(P0.01);对照组和接触组炉底、炉侧、炉顶亚组工作场所空气中COE的CSTEL分别为0.020~0.070(0.042±0.018)、0.100~0.160(0.132±0.023)、0.180~0.300(0.223±0.040)、0.190~0.320(0.269±0.049)mg/m3,呈从低到高趋势(P0.01)。接触组人群尿中1-OHP和8-OHd G水平均高于对照组[0.466(0.160,1.420)vs 0.026(0.011,0.110)μmol/mol肌酐,237.990(128.727,458.055)vs 160.000(94.578,274.256)μg/mol肌酐,P0.01]。研究对象尿中1-OHP水平从低到高依次为对照组接触组炉底亚组接触组炉侧亚组接触组炉顶亚组(P0.05)。多重线性回归分析结果显示,在排除性别、年龄、工龄、吸烟、饮酒和体质量超重等混杂因素的影响后,尿中1-OHP水平和8-OHd G水平均与COE接触有关联(P0.05);接触组人群COE接触水平与尿中1-OHP水平呈剂量-效应关系(P0.01),尿中1-OHP水平与8-OHd G水平呈剂量-效应关系(P0.01)。结论低水平COE接触仍可导致焦炉作业工人DNA氧化损伤,其COE接触水平与DNA氧化损伤标志物尿中8-OHd G水平呈剂量-效应关系。
[Abstract]:Objective to investigate the dose-effect relationship between low level (COE) exposure and urinary 8-hydroxydeoxyguanosine (8-OHd G) in coke oven workers. Methods A cross-sectional investigation was carried out with 263 coke oven workers in a coking plant and 113 non-coke oven workers in an oxygen plant as the control group. Detection of COE Short-term contact concentration (CSTEL). In Workplace Air of two groups The levels of 1-hydroxypyrene (1-OHP) and 8-OHd G in urine were determined by high performance liquid chromatography (HPLC) and ultra high performance liquid chromatography-mass spectrometry respectively. Results the CSTEL of COE in the workplace air of the control group and the exposure group were 0.020 卤0.018 (0.042 卤0.018) and 0.100 卤0.320 (0.208 卤0.069) mg/m3;, respectively. The CSTEL of the workplace air in the exposed group was higher than that in the control group (P0.01). The CSTEL of COE in the bottom of the furnace, the side of the furnace and the subgroup of the furnace top in the control group and the contact group were 0.020 ~ 0.070 (0.042 卤0.018), 0.100 ~ 0.160 (0.132 卤0.023), 0.180 ~ 0.300 (0.223 卤0.040), respectively. (0.269 卤0.049) mg/m3, showed a trend from low to high (P0.01). The levels of 1-OHP and 8-OHd G in urine of the exposed group were higher than those of the control group [0.466 (0.160 卤1.420) vs 0.026 (0.011 卤0.110) 渭 mol/mol creatinine]. 237.990 (128.727458.055) vs 160.000 (94.578274.256) 渭 g/mol creatinine, P0.01]. The levels of 1-OHP in urine were in order from low to high in the control group, the bottom subgroup, the furnace side subgroup, the top subgroup (P0.05). Multiple linear regression analysis showed that the levels of 1-OHP and 8-OHd G in urine were correlated with COE exposure after the factors of sex, age, length of service, smoking, alcohol consumption and overweight were excluded (P0.05). There was a dose-effect relationship between COE exposure level and 1-OHP level in urine (P0.01), and a dose-effect relationship between 1-OHP level and 8-OHd G level in urine (P0.01). Conclusion low level of COE exposure can still lead to DNA oxidative damage in coke oven workers. The COE exposure level is dose-dependent with the urinary 8-OHd G level of DNA oxidative damage marker.
【作者单位】：中国疾病预防控制中心职业卫生与中毒控制所;济南市疾病预防控制中心;莱芜钢铁集团有限公司医院;山东省职业卫生与职业病防治研究院;
【基金】：国家科技支撑计划(2014BAI12B02) 国家自然科学基金(81172642)
【分类号】：R114

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