杨梅酮对高脂膳食小鼠学习记忆能力的改善作用
发布时间：2018-12-06 20:42
【摘要】：目的研究杨梅酮(myricetin,MTN)对高脂膳食小鼠学习记忆能力的影响。方法 64只C57BL/6J雄性小鼠随机分为对照组(CN,基础饲料),杨梅酮对照组(MTN,基础饲料+0.08%杨梅酮),高脂组(HF,高脂饲料),高脂+0.08%杨梅酮组(HF+MTN)4组。6w后采用Morris水迷宫测定小鼠空间学习记忆能力。以基因芯片分别探究高脂膳食与杨梅酮对海马信号通路的影响,q PCR法验证基因芯片结果。检测小鼠海马的氧化应激指标、炎性反应指标和BDNF的水平和相对表达量。结果与CN组相比,HF组小鼠学习记忆能力和BDNF水平显著下降(P0.05);海马SOD和GSH-Px活性显著下降,MDA水平显著升高(P0.05),但TNF-α、IL-6和MCP-1水平无显著变化(P0.05)。长时程增强信号通路和过氧化物酶体信号通路是高脂膳食和杨梅酮共同改变的信号通路(P0.05),高脂诱导海马Sod2、Prdx1、Prdx5、Creb、Itpr1和Plcb3表达显著下降。HF+MTN组认知障碍显著改善,海马SOD活性显著升高,MDA水平显著降低,显著上调Prdx1、Prdx5、Creb和Itpr1的表达(P0.05)。结论杨梅酮通过维持海马氧化还原状态,调节长时程增强信号通路,可改善小鼠的学习记忆能力。
[Abstract]:Objective to study the effect of myricetin (myricetin,MTN) on learning and memory ability in high fat diet mice. Methods 64 male C57BL/6J mice were randomly divided into three groups: control group (CN, basic diet), myricetin control group (MTN, basic diet 0.08% myriceone) and high fat group (HF, high fat diet). After 6 weeks, the spatial learning and memory ability of mice was measured by Morris water maze. The effects of high fat diet and myricetin on hippocampal signal pathway were investigated by gene chip, q PCR method. The levels and relative expression of BDNF and oxidative stress index, inflammatory response index and BDNF in the hippocampus of mice were measured. Results compared with CN group, the ability of learning and memory and the level of BDNF in HF group decreased significantly (P0.05). The activities of SOD and GSH-Px in hippocampus decreased significantly, and the level of MDA increased significantly (P0.05), but the levels of TNF- 伪, IL-6 and MCP-1 did not change significantly (P0.05). Long-term potentiation signal pathway and peroxisome signal pathway were the signal pathways of hyperlipidemia diet and myricetin (P0.05), and hyperlipidemia induced hippocampal Sod2,Prdx1,Prdx5,Creb,. The expression of Itpr1 and Plcb3 decreased significantly in. HF MTN group. In. HF MTN group, the cognitive impairment, the activity of SOD and the level of MDA in hippocampus were significantly increased, and the expression of Prdx1,Prdx5,Creb and Itpr1 were significantly up-regulated (P0.05). Conclusion Myricetin can improve the learning and memory ability of mice by maintaining the redox state of hippocampus and regulating the long term enhancement of signal pathway.
【作者单位】： 江南大学无锡医学院;江南大学食品科学与技术国家重点实验室;中粮集团油脂部;
【基金】：中央高校基本科研业务费专项资金资助(No.JUSRP115A33)
【分类号】：R151
本文编号：2366628
[Abstract]:Objective to study the effect of myricetin (myricetin,MTN) on learning and memory ability in high fat diet mice. Methods 64 male C57BL/6J mice were randomly divided into three groups: control group (CN, basic diet), myricetin control group (MTN, basic diet 0.08% myriceone) and high fat group (HF, high fat diet). After 6 weeks, the spatial learning and memory ability of mice was measured by Morris water maze. The effects of high fat diet and myricetin on hippocampal signal pathway were investigated by gene chip, q PCR method. The levels and relative expression of BDNF and oxidative stress index, inflammatory response index and BDNF in the hippocampus of mice were measured. Results compared with CN group, the ability of learning and memory and the level of BDNF in HF group decreased significantly (P0.05). The activities of SOD and GSH-Px in hippocampus decreased significantly, and the level of MDA increased significantly (P0.05), but the levels of TNF- 伪, IL-6 and MCP-1 did not change significantly (P0.05). Long-term potentiation signal pathway and peroxisome signal pathway were the signal pathways of hyperlipidemia diet and myricetin (P0.05), and hyperlipidemia induced hippocampal Sod2,Prdx1,Prdx5,Creb,. The expression of Itpr1 and Plcb3 decreased significantly in. HF MTN group. In. HF MTN group, the cognitive impairment, the activity of SOD and the level of MDA in hippocampus were significantly increased, and the expression of Prdx1,Prdx5,Creb and Itpr1 were significantly up-regulated (P0.05). Conclusion Myricetin can improve the learning and memory ability of mice by maintaining the redox state of hippocampus and regulating the long term enhancement of signal pathway.
【作者单位】： 江南大学无锡医学院;江南大学食品科学与技术国家重点实验室;中粮集团油脂部;
【基金】：中央高校基本科研业务费专项资金资助(No.JUSRP115A33)
【分类号】：R151
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