基于阳离子膜滤芯的环境水体病毒浓缩方法效果评价

发布时间：2018-12-11 07:46

【摘要】：目的本研究评价和优化基于阳离子膜滤芯的环境水体病毒浓缩方法,为监测环境水体中的病毒提供方法依据。方法采用基于阳离子膜滤芯Nano Ceram的膜吸附洗脱法作为初级浓缩手段,结合有机絮凝和超滤法,从大体积初始水量中浓缩目标病毒。预实验评价不同有机絮凝条件对病毒基因拷贝数回收和病毒滴度的影响。结果随着有机絮凝牛肉浸膏洗脱液p H值的上升,回收病毒的基因拷贝数存在下降趋势(β=41.59,P0.01)。采用2种浓缩流程回收4种水体中的目标病毒,成组t检验显示两者差异有统计学意义(t=2.261,P0.05)。当调节有机絮凝洗脱液p H值为2.0时,水源水中脊髓灰质炎病毒的回收率最高达到7.87%。结论本研究建立的方法可作为今后环境水体常规监测的有效手段。
[Abstract]:Objective to evaluate and optimize the method of virus concentration in environmental water based on cationic membrane filter in order to provide a basis for monitoring viruses in environmental water. Methods the method of membrane adsorption and elution based on cationic membrane filter element (Nano Ceram) was used as the primary concentration method combined with organic flocculation and ultrafiltration to concentrate the target virus from the initial volume of water. The effects of different organic flocculation conditions on viral gene copy number recovery and virus titer were evaluated. Results with the increase of pH value of organic flocculated beef extract, the copy number of recovered virus gene decreased (尾 = 41.59% P0.01). Four kinds of target viruses in water were recovered by two concentration processes. T test showed that there was significant difference between the two groups (P 0.05). When the pH value of organic flocculant eluate is 2.0, the recovery rate of poliovirus in source water reaches 7.87%. Conclusion the method established in this study can be used as an effective method for routine monitoring of environmental water in the future.
【作者单位】：上海市疾病预防控制中心;复旦大学上海医学院医学分子病毒重点实验室;
【基金】：上海市卫生局青年科研项目(编号:20124Y165) 公共卫生三年行动计划优秀学科带头人(编号:GWDTR201201)
【分类号】：R123.1

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