六氟化硫分解产物的皮肤防护物致敏性研究

发布时间：2018-12-13 13:23

【摘要】：目的探讨用于六氟化硫(SF6)分解产物的皮肤防护物对豚鼠皮肤的致敏作用。方法选用体重200~300g普通级Dunkin Hartley雄性豚鼠,以葡萄糖酸钙(5%)+碳酸氢钠(0.5%)混合物为皮肤防护物,以无菌蒸馏水为阴性对照,肉桂醛为阳性对照,采用局部封闭涂皮试验(BT法)和豚鼠最大值试验(GPMT)方法,于实验第1天分别对豚鼠皮肤封闭涂敷和皮内注射各组溶液进行诱导接触,BT法豚鼠在第7、14天进行同样皮肤封闭涂敷诱导,GPMT法豚鼠于第7天采用局部封闭涂皮进行诱导接触,均在末次诱导致敏后第14天进行皮肤涂敷激发接触,观察激发接触后24、48、72h的皮肤过敏反应情况。结果皮肤防护物BT法检测致敏率为10.00%,GPMT法检测致敏率为9.09%,致敏阳性反应的豚鼠皮肤可见散在或小块红斑;肉桂醛BT法致敏率为50.00%,GPMT法致敏率为100.00%,致敏阳性反应的豚鼠皮肤可见中度至重度的融合红斑、轻中度水肿;蒸馏水组豚鼠皮肤无致敏阳性反应,皮肤表现与正常皮肤基本一致。结论葡萄糖酸钙(5%)+碳酸氢钠(0.5%)混合物作为SF6分解产物的皮肤防护物,可使豚鼠皮肤发生轻微的过敏性反应,为轻度致敏物。
[Abstract]:Objective to study the sensitizing effect of skin protectants used in decomposition products of sulfur hexafluoride (SF6) on guinea pig skin. Methods the male guinea pigs of general grade Dunkin Hartley were selected. The mixture of calcium gluconate (5%) sodium bicarbonate (0.5%) was used as skin protector, aseptic distilled water as negative control and cinnamaldehyde as positive control. The local seal coating test (BT) and the guinea pig maximum value test (GPMT) were used to induce contact with each group of solution on the first day of the experiment. BT guinea pigs were induced by the same skin sealing coating on the 7th day, and the GPMT method guinea pigs were induced by local blocking coating on the 7th day, and on the 14th day after the last sensitization, all the guinea pigs were induced by skin coating and excited contact. The skin anaphylaxis was observed at 24 minutes and 48 minutes after exposure. Results the sensitizing rate of skin protector BT was 10.00% and 9.09% respectively. Erythema could be seen in the skin of guinea pigs with positive skin sensitizing reaction. The sensitizing rate of cinnamaldehyde BT method was 50.00g / g. The sensitizing rate of BT method was 100.000.The skin of guinea pigs with positive allergic reaction could be seen to have moderate to severe erythema fusion and mild to moderate edema. In distilled water group, there was no sensitizing reaction in guinea pig skin, and the skin appearance was basically consistent with that of normal skin. Conclusion the mixture of calcium gluconate (5%) sodium bicarbonate (0.5%) as skin protectant of SF6 decomposition product can cause mild allergic reaction in guinea pig skin and is a mild sensitizer.
【作者单位】：武汉科技大学医学院公共卫生学院;国家电网湖北省检修公司;华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系;
【基金】：湖北省自然科学基金资助项目(No.2014CFB11) 武汉科技大学青年科技骨干培育计划资助项目(No.2014XZ032)
【分类号】：R114

【参考文献】