CASP3和CASP7基因多态性与噪声性听力损失易感性关系
发布时间：2018-12-27 20:25
【摘要】：目的探讨细胞凋亡通路相关基因CASP3和CASP7多态性与中国汉族人群噪声性听力损失(NIHL)易感性之间的关联。方法研究对象来自2014年杭州市1 549名噪声接触工人听力损失的横断面调查,采用1:1配对病例对照研究,病例组为电测听双耳高频平均听阈25 d B(A)的工人,对照组为性别、年龄、接噪工龄、工作岗位与病例匹配且双耳所有频段听阈均≤25 d B(A)的工人,共272对。PCR-LDR法检测2个SNP位点的基因型。采用多因素条件logistic回归模型分析SNP位点与NIHL的关联,并以叉生分析计算基因-环境交互作用。结果χ~2检验分析发现,CASP3基因rs1049216等位基因(C、T)频率组间分布有统计学差异(OR=0.68,95%CI=0.50~0.93),而基因型频率组间差异无统计学意义(P0.05);CASP7基因rs10787498等位基因及基因型频率组间分布均无统计学意义(P0.05);多因素条件logistic回归显示,CASP3基因rs1049216中,与野生基因型CC相比,突变基因型(CT+TT)为NIHL的保护因素(调整OR=0.65,95%CI=0.43~0.97);叉生分析表明rs1049216位点与文化程度、睡眠时间存在交互作用(P≤0.001),NIHL的危险性降低(OR值变小)。结论 CASP3基因rs1049216位点可能与中国汉族人群NIHL易感性有关,且可能与文化程度、睡眠时间存在交互作用。
[Abstract]:Objective to investigate the association between the polymorphisms of CASP3 and CASP7 genes associated with apoptosis pathway and the susceptibility to noise-induced hearing loss (NIHL) in Chinese Han population. Methods A cross-sectional investigation of hearing loss among 1 549 noise exposed workers in Hangzhou in 2014 was carried out. A 1:1 matched case-control study was conducted. In the control group, there were 272 pairs of workers who had sex, age, length of exposure to noise, job matching with case and hearing threshold of less than 25 d B (A) at all frequencies. The genotypes of two SNP loci were detected by PCR-LDR method. Multivariate conditional logistic regression model was used to analyze the relationship between SNP locus and NIHL, and the interaction between gene and environment was calculated by cross analysis. Results 蠂 ~ 2 test showed that the distribution of rs1049216 allele (CITT) frequency of CASP3 gene was significantly different (OR=0.68,95%CI=0.50~0.93), but there was no significant difference between genotype frequency groups (P0.05). The distribution of rs10787498 allele and genotype frequency of CASP7 gene were not statistically significant (P0.05). Multivariate conditional logistic regression showed that the mutant (CT TT) was the protective factor of NIHL (adjusted OR=0.65,95%CI=0.43~0.97) in the rs1049216 of CASP3 gene compared with the wild genotype CC. The permutation analysis showed that there was interaction between rs1049216 locus and education level and sleep time (P 鈮，
本文编号：2393559
[Abstract]:Objective to investigate the association between the polymorphisms of CASP3 and CASP7 genes associated with apoptosis pathway and the susceptibility to noise-induced hearing loss (NIHL) in Chinese Han population. Methods A cross-sectional investigation of hearing loss among 1 549 noise exposed workers in Hangzhou in 2014 was carried out. A 1:1 matched case-control study was conducted. In the control group, there were 272 pairs of workers who had sex, age, length of exposure to noise, job matching with case and hearing threshold of less than 25 d B (A) at all frequencies. The genotypes of two SNP loci were detected by PCR-LDR method. Multivariate conditional logistic regression model was used to analyze the relationship between SNP locus and NIHL, and the interaction between gene and environment was calculated by cross analysis. Results 蠂 ~ 2 test showed that the distribution of rs1049216 allele (CITT) frequency of CASP3 gene was significantly different (OR=0.68,95%CI=0.50~0.93), but there was no significant difference between genotype frequency groups (P0.05). The distribution of rs10787498 allele and genotype frequency of CASP7 gene were not statistically significant (P0.05). Multivariate conditional logistic regression showed that the mutant (CT TT) was the protective factor of NIHL (adjusted OR=0.65,95%CI=0.43~0.97) in the rs1049216 of CASP3 gene compared with the wild genotype CC. The permutation analysis showed that there was interaction between rs1049216 locus and education level and sleep time (P 鈮，
本文编号：2393559
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