甲基汞致秀丽隐杆线虫神经毒性作用研究

发布时间：2018-12-31 17:15

【摘要】：目的研究甲基汞对秀丽隐杆线虫神经元的损伤作用,观察线虫虫期、染毒方式、检测指标与时间对甲基汞神经毒性的影响。方法 L1期、L4期秀丽隐杆线虫用0~300μmol/L甲基汞液体染毒30 min或固体染毒24~72 h,于染毒开始后的24~72 h检测神经元形态及行为学指标。结果甲基汞破坏秀丽隐杆线虫多巴胺神经元与γ-氨基丁酸神经元结构的完整性;不论选用L1期还是L4期线虫,采用固体染毒还是液体染毒,甲基汞染毒后24 h即可观察到线虫神经元结构、行为功能显著改变;L1期线虫对甲基汞的毒性作用较L4期线虫敏感;身体弯曲频率、头部摆动频率是对损伤最为敏感的行为学指标。结论甲基汞损伤秀丽隐杆线虫多巴胺神经元与γ-氨基丁酸神经元;秀丽隐杆线虫能够作为甲基汞神经毒性及机制研究的模式生物。
[Abstract]:Objective to study the effects of methylmercury on the neuronal damage of cryptospira elegans, and to observe the effects of nematode stage, exposure mode, detection index and time on the neurotoxicity of methylmercury. Methods in phase L1 and L4, nematodes were exposed to methylmercury for 30 min or solid solution for 2472 hours. The morphology and behavior of neurons were measured at 2472 hours after exposure. Results methylmercury destroyed the structural integrity of dopaminergic and 纬 -aminobutyric acid neurons in C. elegans. The structure and behavioral function of nematode neurons were observed 24 hours after exposure to methylmercury, and the toxicity of nematode in phase L1 to methylmercury was more sensitive than that of nematode in stage L4. The frequency of body bending and the frequency of head swinging are the most sensitive behavioral indicators to injury. Conclusion methylmercury injures dopamine neurons and 纬 -aminobutyric acid neurons of nematode, which can be used as a model organism for the study of neurotoxicity and mechanism of methylmercury.
【作者单位】：华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室;
【基金】：中央高校基本科研业务费专项资金(2013TS011) 国家高科技研究发展计划(2013AA065204)
【分类号】：R114

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