成都市售辣椒中产黄曲霉毒素菌株的分离及其基因分析

发布时间：2018-12-31 20:50

【摘要】：目的从成都市售辣椒粉中,分离产黄曲霉毒素的菌株,探究产毒基因aflR、omt-1和ver-1与产黄曲霉毒素B1(AFB1)能力的关系。方法采用传统培养法分离菌株,多重PCR方法筛查潜在产黄曲霉毒素菌株,对潜在产黄曲霉毒素菌株进行产毒培养,以ELISA方法检测潜在产毒株7d产毒培养液中AFB1含量;同时基于潜在产毒株的产毒关键基因aflR、omt-1和ver-1构建系统进化树,分析其同源性。结果 64份辣椒样品中,分离到17株潜在产AFB1的黄曲霉菌株,有11株分离株产毒,产毒黄曲霉分离率为64.71%;且基于aflR、omt-1和ver-1基因与产毒标准菌株有较高同源性;有6株分离株不产毒,与米曲霉序列有较高同源性。结论控制黄曲霉污染是黄曲霉控制中重要的环节之一。并非含有产毒基因的黄曲霉都会产AFB1毒素,产AFB1能力与产毒基因aflR的序列有着直接联系,而AFB1合成量的高低与omt-1和ver-1基因有关。
[Abstract]:Objective to isolate aflatoxin producing strains from pepper powder in Chengdu, and to explore the relationship between aflatoxin B1 (AFB1) and aflatoxin B1 (AFB1). Methods the strains were isolated by the traditional culture method. The potential aflatoxin producing strains were screened by multiple PCR method. The AFB1 content in the culture medium of the potential aflatoxin producing strains was detected by ELISA method. At the same time, phylogenetic tree was constructed based on the key genes aflR,omt-1 and ver-1 of potential virulence strain, and its homology was analyzed. Results 17 strains of Aspergillus flavus with potential AFB1 production were isolated from 64 pepper samples, 11 strains produced toxin and the isolation rate of Aspergillus flavus was 64.71%, and there was a high homology between Aspergillus flavus strain and standard strain based on aflR,omt-1 and ver-1 genes. There were 6 isolates with high homology with Aspergillus oryzae. Conclusion Aspergillus flavus pollution control is one of the important links in Aspergillus flavus control. Not all Aspergillus flavus with toxic genes can produce AFB1 toxin. The ability to produce AFB1 is directly related to the sequence of aflR, and the amount of AFB1 synthesis is related to omt-1 and ver-1 genes.
【作者单位】：四川大学华西公共卫生学院卫生检验与检疫系;四川省食品安全监测与风险评估重点实验室;
【基金】：国家自然科学基金重点项目(No.81030053)资助
【分类号】：R155.5

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