三氧化二砷对小鼠成体神经发生影响的研究
发布时间：2019-01-03 19:59
【摘要】：目前成体神经干细胞在临床应用中具有广阔的前景,对它的研究一直是国内外神经领域研究的热点,大量的形态学和行为学实验表明成体干细胞与学习记忆密切相关。近年来砷中毒对中枢神经系统的损害已引起人们的高度重视,而有关慢性砷中毒成年小鼠动物模型成体神经发生的研究尚未见报道。本实验通过建立慢性砷中毒小鼠模型,着重应用免疫组化技术在细胞水平上研究三氧化二砷中毒对海马结构中的齿状回颗粒下层(sub granular zone, SGZ区)的成体神经发生的影响,包括成体干细胞的增殖速度和新生细胞的存活,为阐明成体神经发生与慢性砷中毒及学习和记忆的关系提供必要的理论和实验基础。目的：探讨三氧化二砷对小鼠海马成体神经发生的影响。方法：用C57小鼠建立砷中毒模型,和BrdU(5-溴脱氧尿嘧啶核苷)腹腔注射、活体灌注取脑、常规冰冻切片等方法,并用BrdU免疫组化染色结合普通光学显微镜进行显微观察和照相,计数小鼠海马单位长度齿状回内颗粒细胞层中BrdU阳性细胞数并进行统计学分析。结果：BrdU注射后存活24小时处死的空白对照组(NS) BrdU阳性细胞平均数是17.63个,低剂量组(1mg/kg)BrdU阳性细胞平均数是21.17个,高剂量组(2mg/kg) BrdU阳性细胞平均数是11.50个；BrdU注射后存活28天处死的空白对照组(NS) BrdU阳性细胞平均数是11.40个,低剂量组(1mg/kg) BrdU阳性细胞平均数是5.40个,高剂量组(2mg/kg) BrdU阳性细胞平均数是5.57个。结论：三氧化二砷在一定的浓度范围内,可引起海马齿状回成体神经干细胞数量的增加,促进其成体神经发生,但其会抑制新生细胞的存活。
[Abstract]:At present, adult neural stem cells have a broad prospect in clinical application. The research on adult neural stem cells has always been a hot spot in the field of nerve research at home and abroad. A large number of morphological and behavioral experiments show that adult neural stem cells are closely related to learning and memory. In recent years, the damage of arsenic poisoning to the central nervous system has attracted great attention, but the study on adult neurogenesis of adult mice model of chronic arsenic poisoning has not been reported. In this study, we established a model of chronic arsenism in mice and studied the effects of arsenic trioxide on the neurogenesis of adult neurons in the subgranular (sub granular zone, SGZ region of dentate gyrus in the hippocampal formation by immunohistochemical technique at the cellular level. The proliferation rate of adult stem cells and the survival of newborn cells provide the necessary theoretical and experimental basis for elucidating the relationship between adult neurogenesis and chronic arsenism, learning and memory. Objective: to investigate the effect of arsenic trioxide on the development of adult hippocampal nerve in mice. Methods: the model of arsenic poisoning in C57 mice was established, and BrdU (5-bromodeoxyuridine) was injected intraperitoneally. The BrdU positive cells in the granular cell layer of the dentate gyrus of the hippocampal unit length of mice were counted and statistically analyzed by BrdU immunohistochemical staining and light microscope. Results: the average number of (NS) BrdU positive cells in the blank control group was 17.63, and that in the low dose group (1mg/kg) was 21.17. The average number of BrdU positive cells in high dose group (2mg/kg) was 11.50; The average number of (NS) BrdU positive cells in the blank control group was 11.40, and that in the low dose group (1mg/kg) was 5.40. The average number of BrdU positive cells in high dose group (2mg/kg) was 5.57. Conclusion: arsenic trioxide can increase the number of adult neural stem cells in dentate gyrus of sea horse in a certain concentration range, and promote the development of adult nerve, but it can inhibit the survival of newborn cells.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
本文编号：2399812
[Abstract]:At present, adult neural stem cells have a broad prospect in clinical application. The research on adult neural stem cells has always been a hot spot in the field of nerve research at home and abroad. A large number of morphological and behavioral experiments show that adult neural stem cells are closely related to learning and memory. In recent years, the damage of arsenic poisoning to the central nervous system has attracted great attention, but the study on adult neurogenesis of adult mice model of chronic arsenic poisoning has not been reported. In this study, we established a model of chronic arsenism in mice and studied the effects of arsenic trioxide on the neurogenesis of adult neurons in the subgranular (sub granular zone, SGZ region of dentate gyrus in the hippocampal formation by immunohistochemical technique at the cellular level. The proliferation rate of adult stem cells and the survival of newborn cells provide the necessary theoretical and experimental basis for elucidating the relationship between adult neurogenesis and chronic arsenism, learning and memory. Objective: to investigate the effect of arsenic trioxide on the development of adult hippocampal nerve in mice. Methods: the model of arsenic poisoning in C57 mice was established, and BrdU (5-bromodeoxyuridine) was injected intraperitoneally. The BrdU positive cells in the granular cell layer of the dentate gyrus of the hippocampal unit length of mice were counted and statistically analyzed by BrdU immunohistochemical staining and light microscope. Results: the average number of (NS) BrdU positive cells in the blank control group was 17.63, and that in the low dose group (1mg/kg) was 21.17. The average number of BrdU positive cells in high dose group (2mg/kg) was 11.50; The average number of (NS) BrdU positive cells in the blank control group was 11.40, and that in the low dose group (1mg/kg) was 5.40. The average number of BrdU positive cells in high dose group (2mg/kg) was 5.57. Conclusion: arsenic trioxide can increase the number of adult neural stem cells in dentate gyrus of sea horse in a certain concentration range, and promote the development of adult nerve, but it can inhibit the survival of newborn cells.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
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