珊瑚状猴头菌多糖抗氧化作用研究

发布时间：2019-01-04 12:46

【摘要】：目的:探讨珊瑚状猴头菌多糖的抗氧化作用。方法:体外抗氧化活性以测定其总还原力,对DPPH自由基、O2-·、·OH的清除率,抑制体外脂质过氧化能力为指标;体内抗氧化试验:将48只昆明小鼠,雌、雄各半,随机分为对照组、低剂量组【100 mg/(kg·d)】、中剂量组【200 mg/(kg·d)】、高剂量组【400 mg/(kg·d)】4个组。灌胃30d后,采样,测定小鼠血清、心脏、肝脏、肾脏组织中总抗氧化能力、超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶活力及丙二醛含量。结果:随着珊瑚状猴头菌多糖质量浓度的增加,其抗氧化能力增强;当其质量浓度达到2 mg/m L时,还原力吸光度为0.482,对DPPH自由基、O2-·和·OH的清除率分别为80.10%,91.72%和94.33%,脂质过氧化物抑制率为68.71%;可提高心脏、肝脏、肾脏等组织及血清中T-AOC、SOD、GSH-Px和过氧化氢酶活力,降低MDA含量,在高剂量时差异达极显著水平(P0.01)。结论:珊瑚状猴头菌多糖具有抗氧化作用。
[Abstract]:Objective: to study the anti-oxidation effect of hericium coralli polysaccharides. Methods: the antioxidant activity in vitro was used to determine the total reduction ability, the scavenging rate of DPPH free radical, O 2 -, OH and the ability of inhibiting lipid peroxidation in vitro. Antioxidation test in vivo: 48 Kunming mice, half female and half male, were randomly divided into control group, low dose group [100 mg/ (kg d)] and middle dose group [200 mg/ (kg d)]. High dose group [400 mg/ (kg d)] 4 groups. The total antioxidant capacity, superoxide dismutase (SOD), glutathione peroxidase (GSH), catalase activity and malondialdehyde (MDA) content in serum, heart, liver and kidney were measured. Results: with the increase of the concentration of polysaccharides from hericium coralli, the antioxidant ability of hericium coralli increased. When the mass concentration reached 2 mg/m / L, the reductive power absorbance was 0.482, the scavenging rates of DPPH free radical, O _ 2- and OH were 80.101.72% and 94.33%, respectively, and the inhibition rate of lipid peroxide was 68.71%. It could increase the activity of GSH-Px and catalase in heart, liver, kidney and serum, and decrease the content of MDA. The difference was significant at high dose (P0.01). Conclusion: the polysaccharide of hericium coralli has anti-oxidation effect.
【作者单位】：山西农业大学食品科学与工程学院;信阳农林学院生物技术系;山西省食用菌工程技术研究中心;山西农业大学资源与环境学院;
【基金】：山西省自然科学基金项目(2010011043-1) 山西省科技基础条件平台建设项目(2010091017) 山西省科技攻关项目(20130311017-3)
【分类号】：R151

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