砒霜厂男性工人外周血淋巴细胞Fas和Bax基因表达水平与尿砷代谢产物的关系

发布时间：2019-01-07 11:42

【摘要】：目的探讨职业性砷暴露人群外周血淋巴细胞中Fas和Bax基因的表达与尿砷代谢产物之间的关系。方法于2011年10月,选取云南某砒霜厂全部43名职业性砷暴露男性工人为暴露组以及23名无砷暴露史男性人群为对照组。测定尿中无机砷、甲基胂酸、二甲基胂酸和外周血淋巴细胞Fas和Bax m RNA的表达水平。结果与对照组比较,砒霜厂工人尿中无机砷、甲基胂酸、二甲基胂酸、总砷及外周血淋巴细胞中Fas和Bax m RNA的表达水平均较高,二级甲基化指数较低,差异均有统计学意义(P0.05)。尿中无机砷、甲基胂酸,二甲基胂酸、总砷与Fas和Bax m RNA表达水平呈正相关(均P0.05),尿砷一级甲基化指数仅与Fas m RNA表达水平呈正相关(P0.05);尿中砷二级甲基化指数与Fas和Bax m RNA表达水平呈负相关(均P0.05)。结论职业性砷暴露工人外周血淋巴细胞中Fas和Bax基因表达升高可能与砷甲基化代谢相关。
[Abstract]:Objective to investigate the relationship between the expression of Fas and Bax genes in peripheral blood lymphocytes and urinary arsenic metabolites in occupational arsenic exposed population. Methods in October 2011, 43 male workers exposed to occupational arsenic in a arsenic factory in Yunnan Province were selected as exposure group and 23 male workers without history of arsenic exposure as control group. The expressions of inorganic arsenic, methylarsonic acid, dimethyl arsonic acid and Fas and Bax m RNA in peripheral blood lymphocytes were determined. Results compared with the control group, the levels of inorganic arsenic, methylarsonic acid, dimethylarsonic acid, total arsenic and Fas and Bax m RNA in peripheral blood lymphocytes of arsenic factory workers were higher, and the secondary methylation index was lower. The difference was statistically significant (P0.05). Urinary inorganic arsenic, methylarsonic acid, dimethylarsonic acid, total arsenic were positively correlated with the expression of Fas and Bax m RNA (P0.05), the urinary arsenic methylation index was only positively correlated with the expression of Fas m RNA (P0.05). The secondary methylation index of arsenic in urine was negatively correlated with the expression of Fas and Bax m RNA (P0.05). Conclusion the increased expression of Fas and Bax genes in peripheral blood lymphocytes of workers exposed to occupational arsenic may be related to arsenic methylation metabolism.
【作者单位】：昆明医科大学公共卫生学院;
【基金】：国家自然科学基金(81460491) 昆明医科大学本科生创新基金(CX201537)
【分类号】：R135.1

【相似文献】