大豆皂甙在小鼠体内抗慢性炎症的活性研究
发布时间：2019-02-17 09:46
【摘要】：研究背景慢性炎症是促进慢性非传染性疾病(NCDs)发生和发展的关键因素之一,运用膳食来源的具有抗炎活性的植物化学物来防治NCDs具有重要实用价值。大豆皂甙(SS)是来源于大豆及其制品中的一类五环三萜类植物化学物,近年来,研究表明SS具有体外抗炎活性。然而,有关不同SS单体的体内抗炎活性鲜见报道,亟待进一步证实。目的通过建立两种小鼠体内慢性炎症模型,研究三种SS单体(SS-A1、SS-A2与SS-I)对炎症标记物的调节作用,旨在弄清它们在小鼠体内的抗慢性炎症活性,为合理利用SS防治NCDs提供理论依据。方法(1)将72只ICR雄性小鼠随机分为6组(12只/组),对照组3组,注射生理盐水;处理组3组,尾静脉间断(1次/周)注射低剂量脂多糖(LPS),分别处理6周、8周和10周,分析炎症标记物含量以确定建立慢性炎症模型的处理时长。(2)将135只ICR雄性小鼠随机分为9组(15只/组),对照组注射生理盐水,第2-9组注射低剂量LPS,共8周,然后分别再用生理盐水、阿司匹林及不同浓度的SS-A1、SS-A2与SS-I灌胃8周,分析炎症标记物含量。(3)将225只C57BL/6J雄性小鼠随机分为对照组(25只)和高脂组(200只),饲喂18周,按体重和肥胖度确定肥胖小鼠,并每组屠宰10只分析炎症标记物含量。(4)将选出的120只肥胖小鼠随机分为8组,每组15只,分别饲喂含阿司匹林以及不同浓度的SS-A1、SS-A2及SS-I的高脂饲料8周。分析炎症标记物。(5)数据采用SPSS 20.0分析,结果以x±SD表示。结果(1)LPS处理8周和10周的ICR小鼠血清或组织(肝、肾、肾周脂肪与睾周脂肪)中的炎症标记物(TNF-α、IL-6、iNOS、COX-2、NO和PGE2)的含量或mRNA表达量均有不同程度升高(p0.05),我们选择8周作为ICR小鼠慢性炎症模型的建立时长。(2)高脂饲料饲喂18周,按体重和肥胖度的双重标准选择120只肥胖小鼠进入后续干预实验;肥胖小鼠血清或组织中的炎症标记物含量或mRNA表达量显著高于对照组小鼠(p0.05)。(3)在两种小鼠慢性炎症模型中,阿司匹林可有效降低血清或组织中炎症标记物含量或mRNA表达量(p0.05);SS-A1、SS-A2与SS-I也可不同程度地降低炎症标记物含量(p0.05),但未发现浓度依赖效应,它们还可增加血清中抗氧化酶SOD和GSH-Px的水平(p0.05);SS与阿司匹林的抗炎活性未发现有规律的高低差异性。结论(1)以 TNF-α、IL-1β、IL-6、iNOS/NO、COX-2/PGE2 作为炎症标记物,低剂量LPS间断尾静脉注射ICR小鼠8周和高脂饲喂C57BL/6J小鼠18周均可诱导建立体内慢性炎症模型。(2)在两种小鼠体内慢性炎症模型中,SS-A1、SS-A2和SS-I均可抑制炎症标记物的含量或mRNA表达量,表明它们在小鼠体内具有抗慢性炎症活性。
[Abstract]:Background chronic inflammation is one of the key factors to promote the occurrence and development of chronic non-communicable disease (NCDs). It is of great practical value to use the anti-inflammatory phytochemicals from dietary sources to prevent and cure NCDs. Soybean saponins (SS) are a class of pentacyclic triterpenoids derived from soybean and its products. In recent years, studies have shown that SS has anti-inflammatory activity in vitro. However, there are few reports about the anti-inflammatory activity of different SS monomers in vivo, which needs to be further confirmed. Objective to study the effects of three kinds of SS monomers (SS-A1,SS-A2 and SS-I) on the regulation of inflammatory markers in mice by establishing two kinds of chronic inflammatory models in order to find out their anti-chronic inflammatory activities in mice. To provide a theoretical basis for the rational use of SS to prevent and cure NCDs. Methods (1) Seventy-two male ICR mice were randomly divided into 6 groups (n = 12) and control group (n = 3). In the treatment group, low dose lipopolysaccharide (LPS),) was injected intermittently (once a week) into caudal vein for 6 weeks, 8 weeks and 10 weeks, respectively. (2) 135 ICR male mice were randomly divided into 9 groups (15 / group), the control group was injected with normal saline, and the 2-9 group was injected with low dose LPS, for 8 weeks. Then they were given saline, aspirin and different concentrations of SS-A1,SS-A2 and SS-I respectively for 8 weeks. (3) 225 male C57BL/6J mice were randomly divided into control group (25) and hyperlipidemia group (200). (4) 120 obese mice were randomly divided into 8 groups, 15 in each group, fed with high fat diet containing aspirin and different concentrations of SS-A1,SS-A2 and SS-I for 8 weeks. (5) the data were analyzed by SPSS 20.0, and the results were expressed as x 卤SD. Results (1) the inflammatory markers (TNF- 伪, IL-6,iNOS,COX-2,) in serum or tissue (liver, perirenal fat and periorchidism fat) of ICR mice treated with LPS for 8 and 10 weeks. The content of NO and PGE2 or the expression of mRNA increased in varying degrees (p0.05). We chose 8 weeks as the time to establish chronic inflammation model of ICR mice. (2) High fat diet was fed for 18 weeks. According to the double standards of body weight and fat degree, 120 obese mice were selected to participate in the follow-up intervention experiment. The content of inflammatory markers or mRNA expression in serum or tissue of obese mice was significantly higher than that in control mice (p0.05). (3). Aspirin could effectively reduce the content of inflammatory markers or the expression of mRNA in serum or tissue (p0.05). SS-A1,SS-A2 and SS-I also decreased the content of inflammatory markers in varying degrees (p0.05), but there was no concentration-dependent effect. They also increased the levels of antioxidant enzymes SOD and GSH-Px in serum (p0.05). There was no regular difference in anti-inflammatory activity between SS and aspirin. Conclusion (1) TNF- 伪, IL-1 尾, IL-6,iNOS/NO,COX-2/PGE2 were used as inflammatory markers. Low dose LPS intermittent tail vein injection of ICR mice for 8 weeks and hyperlipidemia fed to C57BL/6J mice for 18 weeks could induce chronic inflammation models in vivo. (2) in two kinds of chronic inflammatory models in vivo, SS-A1, was used to induce chronic inflammation in mice. Both SS-A2 and SS-I could inhibit the content of inflammatory markers or the expression of mRNA, indicating that they had anti-chronic inflammatory activity in mice.
【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151
[Abstract]:Background chronic inflammation is one of the key factors to promote the occurrence and development of chronic non-communicable disease (NCDs). It is of great practical value to use the anti-inflammatory phytochemicals from dietary sources to prevent and cure NCDs. Soybean saponins (SS) are a class of pentacyclic triterpenoids derived from soybean and its products. In recent years, studies have shown that SS has anti-inflammatory activity in vitro. However, there are few reports about the anti-inflammatory activity of different SS monomers in vivo, which needs to be further confirmed. Objective to study the effects of three kinds of SS monomers (SS-A1,SS-A2 and SS-I) on the regulation of inflammatory markers in mice by establishing two kinds of chronic inflammatory models in order to find out their anti-chronic inflammatory activities in mice. To provide a theoretical basis for the rational use of SS to prevent and cure NCDs. Methods (1) Seventy-two male ICR mice were randomly divided into 6 groups (n = 12) and control group (n = 3). In the treatment group, low dose lipopolysaccharide (LPS),) was injected intermittently (once a week) into caudal vein for 6 weeks, 8 weeks and 10 weeks, respectively. (2) 135 ICR male mice were randomly divided into 9 groups (15 / group), the control group was injected with normal saline, and the 2-9 group was injected with low dose LPS, for 8 weeks. Then they were given saline, aspirin and different concentrations of SS-A1,SS-A2 and SS-I respectively for 8 weeks. (3) 225 male C57BL/6J mice were randomly divided into control group (25) and hyperlipidemia group (200). (4) 120 obese mice were randomly divided into 8 groups, 15 in each group, fed with high fat diet containing aspirin and different concentrations of SS-A1,SS-A2 and SS-I for 8 weeks. (5) the data were analyzed by SPSS 20.0, and the results were expressed as x 卤SD. Results (1) the inflammatory markers (TNF- 伪, IL-6,iNOS,COX-2,) in serum or tissue (liver, perirenal fat and periorchidism fat) of ICR mice treated with LPS for 8 and 10 weeks. The content of NO and PGE2 or the expression of mRNA increased in varying degrees (p0.05). We chose 8 weeks as the time to establish chronic inflammation model of ICR mice. (2) High fat diet was fed for 18 weeks. According to the double standards of body weight and fat degree, 120 obese mice were selected to participate in the follow-up intervention experiment. The content of inflammatory markers or mRNA expression in serum or tissue of obese mice was significantly higher than that in control mice (p0.05). (3). Aspirin could effectively reduce the content of inflammatory markers or the expression of mRNA in serum or tissue (p0.05). SS-A1,SS-A2 and SS-I also decreased the content of inflammatory markers in varying degrees (p0.05), but there was no concentration-dependent effect. They also increased the levels of antioxidant enzymes SOD and GSH-Px in serum (p0.05). There was no regular difference in anti-inflammatory activity between SS and aspirin. Conclusion (1) TNF- 伪, IL-1 尾, IL-6,iNOS/NO,COX-2/PGE2 were used as inflammatory markers. Low dose LPS intermittent tail vein injection of ICR mice for 8 weeks and hyperlipidemia fed to C57BL/6J mice for 18 weeks could induce chronic inflammation models in vivo. (2) in two kinds of chronic inflammatory models in vivo, SS-A1, was used to induce chronic inflammation in mice. Both SS-A2 and SS-I could inhibit the content of inflammatory markers or the expression of mRNA, indicating that they had anti-chronic inflammatory activity in mice.
【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151
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