青春期氰戊菊酯暴露对小鼠海马甾体激素合成酶及雄激素受体和雌激素受体的影响
发布时间：2019-03-12 18:42
【摘要】：目的本课题通过观察青春期暴露氰戊菊酯对小鼠大脑海马甾体激素合成关键酶（stAR, P450scc, CYP17A1,17β-HSD,3β-HSD和CYP19）及甾体激素受体（AR,ERα和ERβ）表达的调控作用，阐明青春期暴露氰戊菊酯产生内分泌干扰效应的分子机理。 方法实验用ICR小鼠从北京维通利华实验动物技术有限公司购买。购买出生后4周ICR小鼠72只（36只雄鼠和36只雌鼠）。体重18-25g左右，雌雄分开并用常规饲料喂食，按照性别与体重顺序编号，随机分笼。分为4组，分别为溶剂对照组、低剂量氰戊菊酯组、中剂量氰戊菊酯组、高剂量氰戊菊酯组，氰戊菊酯浓度分别为0.02mg/kg,0.2mg/kg,2.0mg/kg。实验前动物自由进食标准饲料，维持12小时光照和12小时黑夜的昼夜规律。实验室温度：20-25℃，湿度：50±5%。实验动物适应性饲养1周后，开始按照先前的分组给予灌胃给药，每天按照小鼠体重的1%的量给药一次。每周称一次体重，连续灌胃一个月后，剖杀老鼠。所有实验均需得到安徽医科大学医学伦理委员会批准。取海马用于下列实验：部分海马组织用液氮速冻后-80℃保存，用于实时定量RT-PCR实验；部分海马组织用液氮速冻后-80℃保存，用于蛋白免疫印迹实验。 结果 1.小鼠青春期和成年期阶段均无疾病、感染和死亡现象，体重的差异无统计学意义。 2.各剂量组雄鼠的17β-HSD, P450scc以及（0.2mg/kg，2mg/kg）剂量组AR的蛋白水平与溶剂对照组相比较均显著下调，，差异均有统计学意义。 3.雄性（0.2mg/kg，2mg/kg）剂量组小鼠AR, cyp17a1, cyp19, ERα, ERβ及各剂量组小鼠的p450scc, star的mRNA表达与溶剂对照组相比较均显著下调，差异均有统计学意义。雌性（0.02mg/kg）剂量组小鼠p450scc，ERα和（0.2mg/kg）剂量组小鼠star，cyp19，ERβ的mRNA表达均显著上调，雌性小鼠各剂量组AR的mRNA 表达均明显下调，差异均有统计学意义。结论小鼠青春期暴露氰戊菊酯干扰各剂量组甾体激素合成关键酶以及雄激素受体和雌激素受体的蛋白表达水平和mRNA表达水平，并存在明显的性别差异。
[Abstract]:Objective to investigate the regulatory effects of fenvalerate on the expression of stAR, P450scc (CYP17A1,17 尾-HSD,3 尾-HSD and CYP19) and steroid hormone receptor (AR, ER 伪 and ER 尾) in hippocampus of mice exposed to fenvalerate during puberty. To elucidate the molecular mechanism of endocrine disruptive effect of fenvalerate during puberty exposure. Methods ICR mice were purchased from Beijing Viton Lihua Laboratory Animal Technology Co., Ltd. 72 ICR mice (36 males and 36 females) were purchased 4 weeks after birth. The weight was about 18g / 25g. The male and female were divided into two groups randomly according to the order of sex and body weight. They were divided into 4 groups: solvent control group, low-dose fenvalerate group, middle-dose fenvalerate group, high-dose fenvalerate group. The concentration of fenvalerate was 0.02 mg / kg, 0.2 mg / kg, 2.0 mg / kg, respectively. Before the experiment, animals were free to eat the standard diet, maintaining 12 hours of light and 12 hours of dark day and night. Laboratory temperature: 20 鈩
本文编号：2439055
[Abstract]:Objective to investigate the regulatory effects of fenvalerate on the expression of stAR, P450scc (CYP17A1,17 尾-HSD,3 尾-HSD and CYP19) and steroid hormone receptor (AR, ER 伪 and ER 尾) in hippocampus of mice exposed to fenvalerate during puberty. To elucidate the molecular mechanism of endocrine disruptive effect of fenvalerate during puberty exposure. Methods ICR mice were purchased from Beijing Viton Lihua Laboratory Animal Technology Co., Ltd. 72 ICR mice (36 males and 36 females) were purchased 4 weeks after birth. The weight was about 18g / 25g. The male and female were divided into two groups randomly according to the order of sex and body weight. They were divided into 4 groups: solvent control group, low-dose fenvalerate group, middle-dose fenvalerate group, high-dose fenvalerate group. The concentration of fenvalerate was 0.02 mg / kg, 0.2 mg / kg, 2.0 mg / kg, respectively. Before the experiment, animals were free to eat the standard diet, maintaining 12 hours of light and 12 hours of dark day and night. Laboratory temperature: 20 鈩
本文编号：2439055
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