低水平母源性十溴联苯醚暴露对子鼠学习记忆能力和甲状腺激素影响

发布时间：2019-03-19 11:53

【摘要】：目的观察十溴联苯醚(BDE209)低水平母源性暴露对子一代小鼠学习记忆能力及甲状腺激素分泌的影响。方法无特定病原体级4周龄雌性昆明小鼠自确定交配成功后,随机分为对照组和低、中、高染毒组,每组16只;对照组母鼠灌胃0.01 L/kg体质量花生油,染毒组母鼠根据50、100和300μg/kg体质量灌胃染毒BDE209,1次/d,连续染毒直至子鼠出生21 d,建立妊娠期至哺乳期的暴露模型。染毒结束后,随机选取每组子鼠10只,雌雄各半,观察其体质量及发育状况;采用水迷宫实验检测子鼠的学习记忆能力;采用化学发光免疫分析法测定子鼠血清总三碘甲腺原氨酸(TT3)、游离三碘甲腺原氨酸(FT3)、总四碘甲腺原氨酸(TT4)和游离四碘甲腺原氨酸(FT4)水平;采用比色法测定子鼠海马组织中谷胱甘肽转移酶(GST)和超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平。结果中染毒组子鼠逃避潜伏期长于对照组(P0.05);高染毒组子鼠逃避潜伏期分别长于对照组和低、中染毒组(P0.05);高染毒组子鼠平台象限活动时间和穿越平台次数分别少于对照组和低、中染毒组(P0.05)。随着染毒剂量的增加,子鼠血清中TT3、FT3、TT4和FT4水平逐渐增高(P0.05),海马组织中GST和SOD活力逐渐降低(P0.05),MDA水平逐渐增高(P0.05)。结论低水平母源性BDE209可导致子鼠学习记忆能力下降,并可导致子鼠血清甲状腺激素水平呈剂量依赖性升高,海马组织出现剂量依赖性的氧化应激损伤。
[Abstract]:Aim to observe the effects of low-level maternal exposure to decabromodiphenyl ether (BDE209) on learning and memory ability and thyroid hormone secretion in offspring. Methods after mating successfully, female Kunming mice of 4 weeks of age without specific pathogen were randomly divided into two groups: control group (n = 16) and low, middle and high dose group (n = 16). The female rats in the control group were fed with 0. 01 L/kg body weight peanut oil. According to the body weight of 50100 渭 g / kg and 300 渭 g / kg, the female rats in the control group were intragastrically administrated with BDE209,1 per day for 21 days, and the exposure model from pregnancy to lactation was established. After exposure, 10 male and female rats in each group were randomly selected to observe their body mass and development, and the learning and memory ability of each group was tested by water maze test. The serum levels of total triiodothyronine (TT3), free triiodothyronine (FT3), total tetraiodothyronine (TT4) and free tetraiodothyronine (FT4) were measured by chemiluminescence immunoassay. The activities of glutathione transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA) in hippocampus were measured by colorimetric method. Results the escape latency of the middle exposure group was longer than that of the control group (P0.05), and the escape latency of the high exposure group was longer than that of the control group and low, respectively (P0.05). The time of platform quadrant activity and the times of crossing the platform in the high exposure group were shorter than those in the control group and low, respectively (P0.05). With the increase of exposure dose, the levels of TT3,FT3,TT4 and FT4 in serum increased gradually (P0.05), and the activities of GST and SOD in hippocampus decreased gradually (P0.05), MDA level gradually increased (P0.05). Conclusion low level of maternal BDE209 can decrease the learning and memory ability of offspring and increase the level of serum thyroid hormone in a dose-dependent manner. There is a dose-dependent oxidative stress injury in hippocampus.
【作者单位】：桂林医学院公共卫生学院;桂林医学院组织胚胎学教研室;
【基金】：国家自然科学基金(81560530) 2016年广西自治区大学生科技创新项目(201610601085)
【分类号】：R114

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