苦荞提取物成分分析及不同极性提取物对α-淀粉酶的抑制作用
发布时间：2019-03-21 11:50
【摘要】：采用石油醚、乙酸乙酯与正丁醇萃取获得不同极性的苦荞提取物,采用超高效液相色谱-串联四极杆飞行时间质谱法分析提取物的成分。结果表明苦荞提取物中含有大量的黄酮类物质,主要包括芦丁、槲皮素、异槲皮素及其糖苷等,还含有羟基苯甲酸等酚酸类物质。再以抑制率、抑制类型、荧光猝灭效应实验分析提取物对α-淀粉酶的抑制作用。结果表明,苦荞提取物对α-淀粉酶的抑制作用表现为乙酸乙酯相提取物、正丁醇相提取物与粗提物的结果类似,都有较为显著的抑制作用,而石油醚相提取物则没有明显且规律的抑制效果,可推断出苦荞提取物中各成分共同发挥了对α-淀粉酶活性的抑制作用,且抑制类型为非竞争性抑制。通过荧光猝灭实验得知抑制的机理为苦荞提取物中的成分与α-淀粉酶结合,从而使α-淀粉酶的荧光特性发生静态猝灭。
[Abstract]:The extracts of Tartary buckwheat with different polarity were extracted by petroleum ether, ethyl acetate and n-butanol. The constituents of extracts were analyzed by ultra-high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry. The results showed that the extract of Tartary buckwheat contained a large number of flavonoids, including rutin, quercetin, isoquercetin and its glycosides, as well as phenolic acids such as hydroxybenzoic acid. Then the inhibitory effect of extract on 伪-amylase was analyzed by inhibition rate, type of inhibition and fluorescence quenching effect. The results showed that the inhibitory effect of Tartary buckwheat extract on 伪-amylase was ethyl acetate extract, and the results of n-butanol phase extract were similar to that of crude extract, all had significant inhibitory effect on 伪-amylase, and the inhibitory effect of n-butanol phase extract was similar to that of crude extract. But the petroleum ether phase extract had no obvious and regular inhibitory effect. It can be concluded that the components of Tartary buckwheat extract play a common role in inhibiting the activity of 伪-amylase, and the type of inhibition is non-competitive inhibition. The mechanism of inhibition was that the components of Tartary buckwheat extract combined with 伪-amylase, thus the fluorescence characteristics of 伪-amylase were quenched statically.
【作者单位】： 江南大学食品学院食品科学与技术国家重点实验室;
【基金】：国家自然科学基金面上项目(31471585)
【分类号】：R151
本文编号：2444903
[Abstract]:The extracts of Tartary buckwheat with different polarity were extracted by petroleum ether, ethyl acetate and n-butanol. The constituents of extracts were analyzed by ultra-high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry. The results showed that the extract of Tartary buckwheat contained a large number of flavonoids, including rutin, quercetin, isoquercetin and its glycosides, as well as phenolic acids such as hydroxybenzoic acid. Then the inhibitory effect of extract on 伪-amylase was analyzed by inhibition rate, type of inhibition and fluorescence quenching effect. The results showed that the inhibitory effect of Tartary buckwheat extract on 伪-amylase was ethyl acetate extract, and the results of n-butanol phase extract were similar to that of crude extract, all had significant inhibitory effect on 伪-amylase, and the inhibitory effect of n-butanol phase extract was similar to that of crude extract. But the petroleum ether phase extract had no obvious and regular inhibitory effect. It can be concluded that the components of Tartary buckwheat extract play a common role in inhibiting the activity of 伪-amylase, and the type of inhibition is non-competitive inhibition. The mechanism of inhibition was that the components of Tartary buckwheat extract combined with 伪-amylase, thus the fluorescence characteristics of 伪-amylase were quenched statically.
【作者单位】： 江南大学食品学院食品科学与技术国家重点实验室;
【基金】：国家自然科学基金面上项目(31471585)
【分类号】：R151
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