有机磷与拟除虫菊酯类混配农药对小鼠细胞免疫毒性作用及机制

发布时间：2019-04-01 11:35

【摘要】：目的研究有机磷与拟除虫菊酯类混配农药对小鼠的细胞免疫系统的影响及其机制。方法以GB 2763—2012《食品安全国家标准食品中农药最大残留限量》中规定的每日允许摄入量为基础,将280只健康SPF级Balb/c小鼠按体重随机分成7组,分别为空白对照(生理盐水)组、溶剂对照(玉米油)组和毒死蜱(0.01 mg/kg)、马拉硫磷(0.3 mg/kg)、氯氰菊酯(0.02 mg/kg)、高效氯氟氰菊酯(0.02 mg/kg)及混配农药组(0.01 mg/kg毒死蜱+0.3 mg/kg马拉硫磷+0.02 mg/kg氯氰菊酯+0.02 mg/kg高效氯氟氰菊酯),每组20只,雌雄各半。采用灌胃方式进行染毒,每日1次,连续30 d。测定小鼠外周血白细胞、淋巴细胞、单核细胞计数和脾淋巴细胞转化功能及外周血T淋巴细胞亚群以及小鼠血清Th1细胞因子水平。结果各染毒组小鼠的脾淋巴细胞转化功能及白细胞、淋巴细胞、单核细胞水平与溶剂对照组比较,差异均无统计学意义(P0.05)。与溶剂对照组比较,仅混配农药组雄性小鼠外周血CD8细胞的比例及血清IL-2、IFN-γ、TNF的水平较低,差异均有统计学意义(P0.05);各单一染毒组雄性小鼠外周血CD3、CD4细胞的比例及CD4/CD8值均无明显改变;各染毒组雌性小鼠血清IL-2、IFN-γ、TNF的水平及外周血CD3、CD4、CD8细胞的比例及CD4/CD8值均无明显改变。结论有机磷与拟除虫菊酯类混配农药对雄性小鼠具有细胞免疫毒性作用,其毒性强度高于同等剂量的单一种类农药。
[Abstract]:Aim to study the effect and mechanism of organophosphorus and pyrethroid pesticides on cellular immune system in mice. Methods based on the daily allowable intake (ADI) specified in GB 2763 / 2012 National Standard for Food Safety, Balb/c mice of SPF grade were randomly divided into 7 groups according to their body weight. They were blank control (saline) group, solvent control (corn oil) group and chlorpyrifos (0.01 mg/kg), malathion (0.3 mg/kg), cypermethrin (0.02 mg/kg), respectively. High efficiency cyhalothrin (0.02 mg/kg) and mixed agricultural medicine group (0.01 mg/kg chlorpyrifos 0.3 mg/kg malathion 0.02 mg/kg cypermethrin 0.02 mg/kg high efficiency cyhalothrin), 20 rats in each group, half male and female. Oral administration was performed once a day for 30 days. The peripheral blood leukocyte, lymphocyte, monocyte count, spleen lymphocyte transformation function, T lymphocyte subsets in peripheral blood and serum Th1 cytokine level in mice were measured. Results there was no significant difference in splenic lymphocyte transformation function, leukocyte, lymphocyte and monocyte levels between each group and the solvent control group (P0.05). Compared with the solvent control group, the proportion of peripheral blood CD8 cells and the levels of serum IL-2,IFN- 纬 and TNF in the mixed agromedicine group were lower than those in the control group (P0.05). The percentage of CD3,CD4 cells and the value of CD4/ CD8 in peripheral blood of male mice exposed to each single dose group had no significant change. The levels of serum IL-2,IFN- 纬 and TNF, the proportion of CD3,CD4,CD8 cells in peripheral blood and the value of CD4/ CD8 in female mice were not significantly changed in all groups. Conclusion the mixture of organophosphorus and pyrethroids has a cytotoxic effect on male mice, and its toxicity intensity is higher than that of the same dose of single pesticide.
【作者单位】：天津市疾病预防控制中心毒理室;
【基金】：天津市卫生局科技基金项目(2013KR10)
【分类号】：R114

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