DHA干预对GDM小鼠胎盘脂肪酸转运蛋白基因表达的影响

发布时间：2019-04-03 16:15

【摘要】：目的探讨二十二碳六烯酸(DHA)干预对妊娠期糖尿病(GDM)小鼠胎盘脂肪酸转运蛋白基因表达的影响。方法选取36只小鼠,其中12只作为空白对照(CN),24只腹腔注射链脲霉素建立GDM模型;GDM小鼠随机分为两组:DHA补充组(GDM+DHA)以及对照组(GDM)。于第10天~18天,给予GDM+DHA组灌胃DHA 500 mg/kg,其他两组灌胃等量溶剂,记录各时期3组血糖及体重水平,第18天收集孕鼠血清及胎盘。羟胺法和比色法分别测定母鼠血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)活力水平,RT-PCR法测定胎盘脂肪酸转运蛋白基因表达水平。结果 (1)重复测量资料的方差分析结果显示:不同时间段的孕鼠体重、血糖差异有统计学意义(P0.05);不同组别孕鼠血糖差异有统计学意义(P0.05)。(2)三组小鼠体内SOD活性、GSH-px活力水平差异有统计学意义(P0.05);与CN组比,GDM组小鼠体内SOD和GSH-px活力水平显著降低(P0.05),DHA干预后,小鼠体内SOD活力水平显著升高(P0.05)。(3)与CN组比,GDM下调了过氧化物酶体增殖激活受体γ(PPARγ)mRNA表达,上调脂肪酸结合蛋白(FABP-pm)、脂肪酸转运蛋白-6(FATP-6 mRNA)表达,DHA干预后,下调了FABP-pm、FATP-6 mRNA的表达。结论DHA干预可增加GDM小鼠体内SOD活力,下调胎盘FABPpm、FATP-6 mRNA的表达。
[Abstract]:Objective to investigate the effect of 22 carbohexaenoic acid (DHA) intervention on the expression of fatty acid transporter gene in placenta of (GDM) mice with gestational diabetes mellitus (GDM). Methods 36 mice were selected, 12 of which were used as blank control (CN), 24 mice were injected intraperitoneally with streptozotocin to establish GDM model, GDM mice were randomly divided into two groups: (GDM DHA) supplemented with DHA and (GDM). As control group. From the 10th day to the 18th day, the rats in the GDM DHA group were given DHA 500 mg/kg, and the other two groups were given the same amount of solvent. The blood glucose and body weight of the three groups were recorded. The serum and placenta of the pregnant rats were collected on the 18th day. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in maternal serum were measured by hydroxylamine method and colorimetric method, and the expression level of fatty acid transporter gene in placenta was measured by RT-PCR method. Results (1) the results of ANOVA of repeated measurements showed that the weight of pregnant rats and the difference of blood glucose in different time periods were statistically significant (P0.05). There was significant difference in blood glucose between different groups of pregnant rats (P0.05). (2). There was significant difference in SOD activity and GSH-px activity level among the three groups (P0.05). Compared with CN group, the activity level of SOD and GSH-px in GDM group was significantly lower than that in CN group (P0.05). (3), compared with CN group (P0.05). (3), after intervention with), DHA, the activity level of SOD in mice was significantly higher than that in CN group. GDM down-regulated the expression of peroxisome proliferator-activated receptor 纬 (PPAR 纬) mRNA and up-regulated the expression of fatty acid binding protein (FABP-pm) and fatty acid transporter-6 (FATP-6 mRNA). After DHA intervention, the expression of FABP-pm,FATP-6 mRNA was down-regulated. Conclusion DHA can increase the activity of SOD and down-regulate the expression of placental FABPpm,FATP-6 mRNA in GDM mice.
【作者单位】：安徽医科大学公共卫生学院营养与食品卫生学系;
【基金】：国家自然科学基金(编号:81373011)
【分类号】：R151

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