干巴菌多糖对急性酒精损伤小鼠的抗氧化作用

发布时间：2019-04-16 13:32

【摘要】：目的:研究干巴菌多糖(Refined polysaccharide from Thelephora ganbajun,RPTG)对急性酒精损伤小鼠心脏、脾脏及肾脏的保护作用。方法:经超声波辅助热水浸提及去蛋白得到干巴菌多糖。将小鼠随机分为空白对照组、模型组、阳性对照组、RPTG各剂量组,建立小鼠急性酒精肝损伤模型。取小鼠心脏、脾脏及肾脏,测定其丙二醛(malondialdehyde,MDA)、还原型谷胱甘肽(glutathione,GSH)含量;超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)及过氧化氢酶(catalase,CAT)活性。结果:与模型对照组相比,RPTG高、中、低剂量组均可以显著或极显著提高心脏、脾脏和肾脏的GSH含量、SOD活性、GSH-Px活性、CAT活性(p0.05或p0.01);均可以显著或极显著降低心脏、脾脏和肾脏的MDA含量(p0.05或p0.01)。结论:RPTG对酒精性肝损伤小鼠心脏、脾脏及肾脏具有明显的抗氧化作用。
[Abstract]:Aim: to study the protective effects of dry bacilli polysaccharide (Refined polysaccharide from Thelephora ganbajun,RPTG) on heart, spleen and kidney in mice with acute alcohol injury. Methods: dry bacilli polysaccharide was obtained by ultrasonic assisted hot water leaching and deproteinization. The mice were randomly divided into three groups: blank control group, model group, positive control group and RPTG group. Acute alcoholic liver injury model was established in mice. The contents of malondialdehyde (malondialdehyde,MDA) and glutathione (glutathione,GSH) in heart, spleen and kidney of mice were measured. The activities of superoxide dismutase (superoxide dismutase,SOD), glutathione peroxidase (glutathione peroxidase,GSH-Px) and catalase (catalase,CAT) were measured. Results: compared with the model control group, RPTG significantly increased GSH content, SOD activity, GSH-Px activity and CAT activity in heart, spleen and kidney (p0.05 or p0.01) in both middle and low dose groups. The contents of MDA in heart, spleen and kidney were significantly or extremely significantly decreased (p0.05 or p0.01). Conclusion: RPTG has obvious antioxidation effect on heart, spleen and kidney of mice with alcoholic liver injury.
【作者单位】：南京师范大学金陵女子学院;
【分类号】：R151

【相似文献】