印刷油墨挥发物对小鼠认知行为及脑组织氨基酸类神经递质的影响

发布时间：2019-04-18 10:57

【摘要】：目的探讨印刷油墨挥发物暴露对小鼠认知行为及脑组织中氨基酸类神经递质的影响。方法将30只健康SPF级雄性昆明小鼠随机分为3组,分别为对照组、新闻纸油墨组和铜版纸油墨组,每组10只。采用静式吸入方式进行染毒,新闻纸油墨组、铜版纸油墨组的平均染毒浓度分别为152、270 mg/m~3,每日1次,每次2 h,每周6 d,持续染毒4周。采用水迷宫和旷场试验测试小鼠的认知行为变化,并检测小鼠脑组织中谷氨酸(Glu)、甘氨酸(Gly)、γ-氨基丁酸(GABA)及天冬氨酸(Asp)的含量。结果与对照组相比,新闻纸油墨组和铜版纸油墨组小鼠首次达台时间均延长,目标象限时间均缩短,穿台次数均减少,差异有统计学意义(P0.05);与新闻纸油墨组相比,铜版纸油墨组小鼠首次达台时间均延长,目标象限时间均缩短,穿台次数均减少,差异有统计学意义(P0.05)。与对照组相比,新闻纸油墨组及铜版纸油墨组小鼠在中央格停留时间延长,水平穿越格子数、直立次数均下降,仅铜版纸油墨组小鼠的修饰次数下降,差异均有统计学意义(P0.05)。与新闻纸油墨组相比,铜版纸油墨组小鼠在中央格停留时间延长,水平穿越格子数、直立次数均下降,差异均有统计学意义(P0.05);而修饰次数无明显变化。与对照组相比,新闻纸油墨组及铜版纸油墨组小鼠脑组织Glu、Gly、GABA、Asp的含量均下降,差异均有统计学意义(P0.05)。与新闻纸油墨组相比,铜版纸油墨组小鼠脑组织Glu、GABA、Asp的含量均下降,差异均有统计学意义(P0.05);而Gly的含量无明显变化。结论油墨挥发物暴露可影响小鼠的记忆及探究行为,并可致脑组织氨基酸类神经递质分泌减少。
[Abstract]:Objective to investigate the effects of printing ink volatiles exposure on cognitive behavior and amino acid neurotransmitters in brain tissue of mice. Methods 30 healthy male Kunming mice of SPF grade were randomly divided into three groups: control group, newsprint ink group and copper paper ink group, with 10 mice in each group. The average exposure concentration of newsprint ink group and copperplate paper ink group was 152270 mg/m~3, once a day for 2 hours, 6 days a week for 4 weeks. The cognitive behavior of mice was measured by water maze and open-field test. The contents of glutamate (Glu), glycine, (Gly), 纬-aminobutyric acid (GABA) and aspartic acid (Asp) in brain tissue of mice were measured. Results compared with the control group, the first arrival time of the newsprint ink group and the copperplate paper ink group were prolonged, the target quadrant time was shortened, and the times of wearing the stage were reduced, the difference was statistically significant (P0.05). Compared with the newsprint ink group, the first arrival time of the coated paper ink group was prolonged, the target quadrant time was shortened, and the number of piercing times was reduced, the difference was statistically significant (P0.05). Compared with the control group, the mice in the newsprint ink group and the coated paper ink group had longer staying time in the central lattice, the number of horizontal traversing grids and the number of erect times decreased, and the modification times of only the coated paper ink group decreased. The difference was statistically significant (P0.05). Compared with the newsprint ink group, the mice in the copperplate ink group had longer stay time in the central grid, the number of horizontal traversing the lattice, the number of erect decreased, the difference was statistically significant (P0.05); but the modification times had no significant change (P0.05). Compared with the control group, the contents of Glu,Gly,GABA,Asp in the brain tissue of the newsprint ink group and the coated paper ink group were significantly lower than those of the control group (P0.05). Compared with the newsprint ink group, the content of Glu,GABA,Asp in the brain tissue of the copper-coated paper ink group decreased significantly (P0.05), but the content of Gly did not change significantly. Conclusion exposure to ink volatiles can affect the memory and inquiry behavior of mice and decrease the secretion of amino acid neurotransmitters in brain tissue.
【作者单位】：华北理工大学公共卫生学院;唐山市环境监测中心站;北京工业大学环境与能源学院;
【基金】：国家环境保护部科研专项(201209003)
【分类号】：R114

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