紫薯醋对小鼠急性肝损伤保护及减肥降脂作用

发布时间：2019-05-07 22:20

【摘要】：以紫薯醋为受试物对小鼠进行动物试验,研究紫薯醋的减肥降脂作用及对小鼠CCl4急性肝损伤的保护作用。将30只小鼠随机分成3组,即以基础饲料喂养的空白对照组、仅以高脂饲料喂养的高脂模型组和以高脂饲料与0.01mL/g紫薯醋喂养的紫薯醋试验组。连续喂养30d后,测定小鼠体质量、脂肪质量,以及血清总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)浓度。另将40只小鼠随机分成4组,即以等量纯净水灌胃的空白对照组、以等量纯净水灌胃的模型组、以100mg/(kg·d)联苯双酯悬浮液灌胃的阳性对照组和0.01mL/g紫薯醋灌胃的紫薯醋试验组。连续喂养3周,最后1d给药1h后,除空白对照组腹腔注射10mL/kg花生油外,其他各组腹腔注射含2g/L CCl4花生油10mL/kg,禁食不禁水16h,摘眼球取血,离心取血清,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和总胆红素(TBIL)浓度。取小鼠肝脏制成0.1g/mL匀浆,测定小鼠肝中的丙二醛(MDA)质量摩尔浓度、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性。结果表明,紫薯醋可显著降低小鼠的体质量和脂肪系数,说明紫薯醋可预防高脂饮食引起的肥胖。紫薯醋对小鼠血清ALT、AST活性和TBIL浓度的升高有显著的抑制作用。对小鼠肝组织匀浆相关指标进行测定,发现紫薯醋可降低肝组织的MDA质量摩尔浓度,减轻肝脏的损伤程度,提高小鼠肝脏的GSH-Px活性,说明紫薯醋对CCl4诱导的小鼠急性肝损伤具有较显著的预防效果。
[Abstract]:The effect of purple potato vinegar on reducing weight and lipid and protecting the acute liver injury of CCl4 in mice was studied by using purple potato vinegar as the test material. Thirty mice were randomly divided into three groups: the blank control group fed with basic diet, the high fat model group fed only with high fat diet and the purple potato vinegar test group fed with high fat diet and 0.01mL/g purple potato vinegar. After 30 days of continuous feeding, the body mass, fat mass and serum total cholesterol, (TC), triglyceride, (TG), high density lipoprotein cholesterol (HDL-C) were measured. The other 40 mice were randomly divided into 4 groups: the blank control group was given the same amount of pure water, and the model group was given the same amount of pure water. The 100mg/ (kg 路d) biphenyl diester suspension was administered to the positive control group and the 0.01mL/g purple potato vinegar test group. After 3 weeks of continuous feeding and 1 h of administration on the last day, except for the blank control group, 10mL/kg peanut oil was injected intraperitoneally with 10 mL / kg of 2g/L CCl4 peanut oil, fasting water for 16 hours, blood was drawn from eyeball, and serum was centrifuged. Serum glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST) activity and total bilirubin (TBIL) concentration were measured. The contents of malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the liver of mice were determined by 0.1g/mL homogenate. The results showed that purple potato vinegar could significantly reduce the body mass and fat coefficient of mice, indicating that purple potato vinegar could prevent obesity caused by high fat diet. Purple potato vinegar significantly inhibited the increase of serum ALT,AST activity and TBIL concentration in mice. The relative indexes of liver homogenate of mice were determined. It was found that purple potato vinegar could reduce the molar concentration of MDA in liver tissue, reduce the degree of liver injury, and increase the activity of GSH-Px in mouse liver. The results showed that purple potato vinegar had significant preventive effect on acute liver injury induced by CCl4 in mice.
【作者单位】：西北农林科技大学食品科学与工程学院;
【基金】：西北农林科技大学国家级“大学生科技创新试验计划”(1210712134)
【分类号】：R151

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