亚慢性钒染毒大鼠海马细胞凋亡及学习记忆的改变

发布时间：2019-05-21 21:35

【摘要】：目的观察亚慢性染毒偏钒酸钠对大鼠海马细胞凋亡的影响,分析其与学习记忆损伤的关系,探讨偏钒酸钠神经毒性机制。方法选取健康雄性SD大鼠48只,随机分为空白组及低、中、高剂量组。偏钒酸钠饮水染毒3个月后,Morris水迷宫检测大鼠学习记忆能力;流式细胞仪检测海马细胞凋亡。结果中、高染钒组大鼠平均潜伏期显著高于空白组,高钒组高于低钒组,差异有统计学意义(P0.05);中、高染钒组大鼠穿越平台次数显著低于空白组,差异有统计学意义(P0.05);中、高染钒组海马细胞早期凋亡率和总凋亡率显著高于空白组,差异有统计学意义(P0.05)。Spearman相关性分析结果显示,钒染毒大鼠海马细胞早期凋亡率与平均潜伏期呈正相关(rs=0.761,P0.01),与穿越平台次数呈负相关(rs=-0.502,P0.01);海马细胞总凋亡率与平均潜伏期呈正相关(rs=0.766,P0.01),与穿越平台次数呈负相关(rs=-0.487,P0.01)。结论亚慢性钒中毒可导致大鼠海马细胞凋亡和学习记忆能力损伤,海马细胞凋亡可能是偏钒酸钠诱发大鼠学习记忆能力损伤的机制之一。
[Abstract]:Objective to observe the effect of subchronic exposure to sodium metavadate on apoptosis of hippocampal cells in rats, to analyze the relationship between sodium metavadate and learning and memory impairment, and to explore the neurotoxicity mechanism of sodium metavadate. Methods 48 healthy male SD rats were randomly divided into blank group and low, middle and high dose group. After 3 months of sodium metavanadate drinking water, the learning and memory ability of rats was measured by Morris water maze, and the apoptosis of hippocampal cells was detected by flow cytometry. The results showed that the average latency of rats in high vanadium exposure group was significantly higher than that in blank group, and that in high vanadium group was higher than that in low vanadium group, the difference was statistically significant (P 0.05). In the high vanadium exposure group, the number of rats crossing the platform in the high vanadium exposure group was significantly lower than that in the blank group, the difference was statistically significant (P 0.05). The early apoptosis rate and total apoptosis rate of hippocampal cells in high vanadium exposure group were significantly higher than those in blank group (P 0.05). Spearman correlation analysis showed that The early apoptosis rate of hippocampal cells exposed to vanadium was positively correlated with the average latency (rs=0.761,P0.01) and negatively correlated with the number of crossing the platform (rs=-0.502,P0.01). The total apoptosis rate of hippocampal cells was positively correlated with the average latency (rs=0.766,P0.01) and negatively correlated with the number of crossing the platform (rs=-0.487,P0.01). Conclusion Subchronic vanadium poisoning can lead to apoptosis of hippocampal cells and damage of learning and memory ability in rats. Apoptosis of hippocampal cells may be one of the mechanisms of learning and memory impairment induced by sodium metavanadate in rats.
【作者单位】：遵义医学院公共卫生学院劳动卫生与环境卫生学教研室;
【基金】：贵州省联合基金(CK-1002)
【分类号】：R114

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