对羟基苯甲酸丁酯通过氧化应激导致人精子活力降低和凋亡增加

发布时间：2019-05-24 12:47

【摘要】：目的探究对羟基苯甲酸丁酯(BP)对人精子活力、氧化应激和细胞凋亡的影响及其机制。方法选取人精液样本20份,随机分为4组:对照组及BP低、中、高浓度组,每组5个平行样。与BP体外共培养4 h,BP染毒浓度分别为0、200、400和800μmol/L。观察精子活动力、细胞毒性、活性氧阳性细胞率和细胞凋亡率。结果BP低、中、高浓度组精子总活力[(26.44±7.83)%、(32.79±2.90)%和(10.51±5.88)%]均低于对照组[(47.67±3.93)%],差异有统计学意义(P0.05)。与对照组相比,BP低、中、高浓度组精子存活率分别为(63.36±9.08)%、(49.72±7.15)%和(29.91±5.93)%。BP低、中、高浓度组活性氧阳性细胞率[(24.67±0.50)%、(54.50±3.40)%和(59.93±3.47)%]高于对照组[(8.63±0.57)%],差异有统计学意义(P0.05),晚期精子凋亡率[(11.8±1.74)%、(12.87±0.25)%和(14.60±0.87)%]高于对照组[(9.63±1.00)%],差异有统计学意义(P0.05)。精子活性氧的水平和晚期凋亡率均与精子的总活力呈负相关,r分别为-0.727和-0.688(P0.05)。结论对羟基苯甲酸丁酯具有细胞毒性,能够降低精子活动能力,促进精子活性氧的生成和细胞凋亡。
[Abstract]:Objective to investigate the effects and mechanism of butylp-hydroxybenzoate (BP) on human sperm motility, oxidative stress and apoptosis. Methods Twenty human semen samples were randomly divided into 4 groups: control group and low, middle and high concentration BP groups, with 5 parallel samples in each group. When co-cultured with BP for 4 h, the concentration of BP was 0200400 渭 mol / L and 800 渭 mol / L, respectively. Sperm motility, cytotoxicity, active oxygen positive cell rate and apoptosis rate were observed. Results the total sperm motility in the high concentration group [(26.44 卤7.83)%, (32.79 卤2.90)% and (10.51 卤5.88)%] was lower than that in the control group [(47.67 卤3.93)%], and the difference was statistically significant (P 0.05). Compared with the control group, the sperm survival rate of the middle and high concentration groups was (63.36 卤9.08)%, (49.72 卤7.15)% and (29.91 卤5.93)%, respectively. The rate of active oxygen positive cells in high concentration group [(24.67 卤0.50)%, (54.50 卤3.40)% and (59.93 卤3.47)%] was significantly higher than that in control group [(8.63 卤0.57)%] (P 0.05). The apoptosis rate of late sperm [(11.8 卤1.74)%, (12.87 卤0.25)% and (14.60 卤0.87)%] was significantly higher than that of the control group [(9.63 卤1.00)%] (P 0.05). The level of reactive oxygen species (Ros) and the late apoptosis rate of spermatozoa were negatively correlated with the total motility of spermatozoa, r =-0.727 and-0.688, respectively (P 0.05). Conclusion Butyl p-hydroxybenzoate has cytotoxicity, can reduce sperm motility and promote sperm reactive oxygen species production and apoptosis.
【作者单位】：中国疾病预防控制中心职业卫生与中毒控制所;国家卫生计生委男性生殖健康重点实验室国家人口计生委科学技术研究所;
【基金】：国家自然科学基金(No.30972447)
【分类号】：R114

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