50Hz工频磁场暴露对FL细胞内神经酰胺代谢的影响
发布时间：2019-06-03 13:39
【摘要】：实验目的： 研究工频磁场暴露对FL细胞内神经酰胺代谢的影响。 实验方法： 人羊膜(FL)细胞经50Hz,0.4mT工频磁场分别暴露5min,15min,30min和1hour后,用改良的Folch法进行脂类提取,采用高效液相色谱串联质谱法PIS模式下分析FL细胞内神经酰胺的种类,在MRM模式下分析不同神经酰胺含量变化；随后分别用伏马菌素(FB1)和丙咪嗪(Imipramine)预处理抑制神经酰胺从头合成途径和酸性鞘磷脂酶水解途径,用N-acetylcysteine(NAC)预处理抑制活性氧(ROS)后,以工频磁场暴露15min,并采用同样的方法提取并检测FL细胞内神经酰胺含量的变化。实验结果用SPSS16.0统计软件进行统计分析。 实验结果： 经工频磁场辐照5min,15min,30min和1hour后,细胞内神经酰胺含量明显升高,且C16CER均有统计学意义(1hour组P0.01,其余组P＜0.05),C18CER在5min差异有统计学意义(P0.05)；C24CER在15min(P0.01)和1hour(P0.05)差异有统计学意义。伏马菌素(FB1)预处理可抑制工频磁场暴露对神经酰胺含量的诱导作用,然而没有统计学差异；而丙咪嗪(Imipramine)预处理则能显著抑制工频磁场对C24CER的诱导作用(P0.05),C18CEP啥量虽有有一定程度降低,但未见有统计学差异。NAC预处理同样可以显著抑制工频磁场对C24CER神经酰胺的诱导作用(P0.05)。 结论： 1.50Hz工频磁场暴露可以诱导FL细胞内C16,C18和C24神经酰胺含量的增高。 2.工频磁场暴露诱导FL细胞内神经酰胺含量的增高可能通过酸性鞘磷脂酶的水解途径和从头合成途径。 3.工频磁场诱导神经酰胺含量(C24CER)的升高与细胞内ROS存在一定相关性。
[Abstract]:Objective: to study the effect of power frequency magnetic field exposure on ceramide metabolism in FL cells. Methods: human amniotic membrane (FL) cells were exposed to 50Hz and 0.4mT power frequency magnetic field for 5 min. After 15 min, 30 min and 1hour, the lipids were extracted by modified Folch method. The types of ceramide in FL cells were analyzed by high performance liquid chromatography (HPLC) tandem mass spectrometry (PIS), and the changes of ceramide content in FL cells were analyzed under MRM mode. Subsequently, fumonisin (FB1) and imipramine (Imipramine) pretreatment were used to inhibit ceramide ab initio synthesis pathway and acid sphingomyelase hydrolysis pathway, respectively. After N-acetylcysteine (NAC) pretreatment, reactive oxygen species (ROS) was suppressed and exposed to power frequency magnetic field for 15 min. The changes of ceramide content in FL cells were extracted and detected by the same method. The experimental results were analyzed by SPSS16.0 statistical software. The results showed that after 5 min of power frequency magnetic field irradiation, the content of ceramide in the cells increased significantly at 30 min, 30 min and 1hour, and the content of C16CER was significantly higher than that in the other groups (P0.01 in 1hour group and P < 0.05 in other groups). The difference of C18CER in 5min was statistically significant (P 0.05). There was significant difference in C24CER between 15min (P 0.01) and 1hour (P 0.05). Fumonisin (FB1) pretreatment could inhibit the induction of ceramide content induced by power frequency magnetic field exposure, but there was no significant difference. Imipramine (Imipramine) pretreatment could significantly inhibit the induction of C24CER by power frequency magnetic field (P 0.05). Although the amount of C18CEP decreased to a certain extent, However, there was no significant difference. NAC pretreatment could also significantly inhibit the induction of C24CER ceramide by power frequency magnetic field (P 0.05). Conclusion: 1.50Hz power frequency magnetic field exposure can induce the increase of C 16, C 18 and C 24 ceramide contents in FL cells. two銆，
本文编号：2491972
[Abstract]:Objective: to study the effect of power frequency magnetic field exposure on ceramide metabolism in FL cells. Methods: human amniotic membrane (FL) cells were exposed to 50Hz and 0.4mT power frequency magnetic field for 5 min. After 15 min, 30 min and 1hour, the lipids were extracted by modified Folch method. The types of ceramide in FL cells were analyzed by high performance liquid chromatography (HPLC) tandem mass spectrometry (PIS), and the changes of ceramide content in FL cells were analyzed under MRM mode. Subsequently, fumonisin (FB1) and imipramine (Imipramine) pretreatment were used to inhibit ceramide ab initio synthesis pathway and acid sphingomyelase hydrolysis pathway, respectively. After N-acetylcysteine (NAC) pretreatment, reactive oxygen species (ROS) was suppressed and exposed to power frequency magnetic field for 15 min. The changes of ceramide content in FL cells were extracted and detected by the same method. The experimental results were analyzed by SPSS16.0 statistical software. The results showed that after 5 min of power frequency magnetic field irradiation, the content of ceramide in the cells increased significantly at 30 min, 30 min and 1hour, and the content of C16CER was significantly higher than that in the other groups (P0.01 in 1hour group and P < 0.05 in other groups). The difference of C18CER in 5min was statistically significant (P 0.05). There was significant difference in C24CER between 15min (P 0.01) and 1hour (P 0.05). Fumonisin (FB1) pretreatment could inhibit the induction of ceramide content induced by power frequency magnetic field exposure, but there was no significant difference. Imipramine (Imipramine) pretreatment could significantly inhibit the induction of C24CER by power frequency magnetic field (P 0.05). Although the amount of C18CEP decreased to a certain extent, However, there was no significant difference. NAC pretreatment could also significantly inhibit the induction of C24CER ceramide by power frequency magnetic field (P 0.05). Conclusion: 1.50Hz power frequency magnetic field exposure can induce the increase of C 16, C 18 and C 24 ceramide contents in FL cells. two銆，
本文编号：2491972
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