硫酸铍致小鼠肺线粒体通透性转换孔高通透性开放及细胞凋亡研究
发布时间：2019-06-20 21:58
【摘要】：目的研究腹腔注射硫酸铍(Be SO4·4H2O)溶液致小鼠肺线粒体通透性转换孔(m PTP)的高通透性开放及细胞凋亡效应。方法 6周龄雄性昆明小鼠36只随机分成3组,每组12只:空白对照组、1.0 mg/kg Be SO4组和2.0 mg/kg Be SO4组,分别按0.1 ml/10 g(体重)腹腔注射灭菌生理盐水、1.0、2.0 mg/kg Be SO4灭菌生理盐水,均隔天注射,一月后处死小鼠取肺脏:差速离心制备线粒体悬液检测线粒体活性氧(ROS)、跨膜电位(Δψm)和m PTP开放度;提取蛋白测定细胞色素C(Cyt-c)、Bax、Bcl-2的表达;电子显微镜观察肺细胞超微结构。结果与对照组比较,硫酸铍染毒组小鼠肺线粒体ROS含量、PTP开放度、细胞色素C和Bax表达均增加(P0.05),Δψm、Bcl-2及Bcl-2/Bax比例均降低(P0.05);染毒组小鼠肺上皮细胞绒毛消失,细胞核固缩,凋亡特征明显。结论腹腔注射硫酸铍可致小鼠肺细胞线粒体通透性转换孔高通透性开放及肺细胞凋亡。
[Abstract]:Objective to study the effects of intraabdominal injection of beryllium sulfate (Be SO4 路4H2O) solution on high permeability opening and apoptosis of (m PTP) in mouse lung mitochondrial permeability transition pore. Methods 36 6-week-old male Kunming mice were randomly divided into 3 groups with 12 mice in each group: blank control group, 1.0 mg/kg Be SO4 group and 2.0 mg/kg Be SO4 group, respectively, treated with 0.1 ml/10 g (body weight) intraabdominal injection of sterilized saline and 1.0 mg/kg Be SO4 sterilized saline, all of which were injected with saline every other day. One month later, the mice were killed and the lungs were taken. The mitochondrial suspension was prepared by differential centrifugation to detect the transmembrane potential (螖 (ROS), m) and m PTP openness of reactive oxygen species (Ros). The expression of cytochrome C (Cyt-c) and Bax,Bcl-2 were measured by extracted protein, and the ultrastructure of lung cells was observed by electron microscope. Results compared with the control group, the content of ROS, the openness of PTP, the expression of C and Bax in the lung of mice exposed to beryllium sulfate increased (P 0.05), the ratio of Bcl 鈮，
本文编号：2503540
[Abstract]:Objective to study the effects of intraabdominal injection of beryllium sulfate (Be SO4 路4H2O) solution on high permeability opening and apoptosis of (m PTP) in mouse lung mitochondrial permeability transition pore. Methods 36 6-week-old male Kunming mice were randomly divided into 3 groups with 12 mice in each group: blank control group, 1.0 mg/kg Be SO4 group and 2.0 mg/kg Be SO4 group, respectively, treated with 0.1 ml/10 g (body weight) intraabdominal injection of sterilized saline and 1.0 mg/kg Be SO4 sterilized saline, all of which were injected with saline every other day. One month later, the mice were killed and the lungs were taken. The mitochondrial suspension was prepared by differential centrifugation to detect the transmembrane potential (螖 (ROS), m) and m PTP openness of reactive oxygen species (Ros). The expression of cytochrome C (Cyt-c) and Bax,Bcl-2 were measured by extracted protein, and the ultrastructure of lung cells was observed by electron microscope. Results compared with the control group, the content of ROS, the openness of PTP, the expression of C and Bax in the lung of mice exposed to beryllium sulfate increased (P 0.05), the ratio of Bcl 鈮，
本文编号：2503540
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