六价铬污染地下水对小鼠肝脏和血糖的影响及维生素C的干预作用
发布时间：2019-06-22 07:42
【摘要】：目的 了解六价铬[Cr(Ⅵ)]污染地区地下水对小鼠肝脏、血糖的影响和维生素C（VitC）的干预作用。 方法 将80只健康6～8周龄清洁级雄性昆明小鼠随机分为8组，分别为对照（蒸馏水）组、铬污染的井水（采自某被污染的地下水，Cr(Ⅵ)含量为12.35mg/L）组和34.91（Cr(Ⅵ)含量为12.35mg/L）、349.1、698.2、1396.4mg/L重铬酸钾单独染毒组以及Vit C（100mg/ml）+698.2、1396.4mg/L重铬酸钾联合作用组，每组10只。采用经口灌胃方式进行染毒，重铬酸钾和井水组的染毒容量为25ml/kg；Vit C的染毒容量为5ml/kg，0.5h后再进行重铬酸钾染毒。连续染毒30d。在试验过程中观察小鼠的中毒表现，染毒结束后收集血液和肝组织。血清天门冬氨酸氨基转移酶（AST）、血糖（Glu）含量、谷丙氨酸氨基转移酶（ALT）活力用全自动生化分析仪测定；肝脏组织超氧化物歧化酶（SOD）活力和谷胱甘肽（GSH）、丙二醛（MDA）含量用可见分光光度法检测；HE染色法观察肝细胞病理形态学改变。 结果 井水组和各剂量重铬酸钾染毒组小鼠在染毒过程中出现了较明显的中毒症状。与对照组比较，井水组和不同浓度重铬酸钾单独染毒组小鼠血清AST、ALT活力和肝脏MDA含量均较高，肝脏SOD活力、GSH含量和血清Glu含量较低，差异有统计学意义（P＜0.05）；而经Vit C预处理各组小鼠血清AST、ALT活力和Glu含量及肝脏SOD活力和GSH、MDA含量无显著变化；且随着重铬酸钾染毒浓度的升高，小鼠血清AST、ALT活力及肝脏MDA含量呈上升趋势，，肝脏SOD活力和GSH含量呈下降趋势。与相同浓度重铬酸钾单独染毒组比较，经VitC预处理各组小鼠血清AST、ALT活力和肝脏MDA含量均较低，血清Glu含量及肝脏SOD活力和GSH含量均较高，差异有统计学意义（P＜0.05）。与34.91mg/L重铬酸钾单独染毒组比较，井水组小鼠血清AST、ALT活力和肝脏MDA含量均较高，血清Glu含量及肝脏SOD活力和GSH含量均较低，差异有统计学意义（P＜0.05）。 结论 Cr(Ⅵ)污染地区地下水可引起小鼠肝脏的损伤，且可能存在其他重金属污染；而Vit C对于重铬酸钾所致肝损伤有一定的拮抗作用。
[Abstract]:Objective to investigate the effect of groundwater on liver and blood glucose in mice polluted by hexavalent chromium [Cr (VI)] and the intervention effect of vitamin C (VitC). Methods 80 healthy male Kunming mice of 6 weeks old and 8 weeks old were randomly divided into 8 groups: control group (distilled water) group, chromium contaminated well water (12.35mg/L) group (12.35mg/L) and 34.91 (Cr (VI (12.35mg/L) group, respectively, the control group (distilled water), the chromium contaminated well water (12.35mg/L) group and 34.91 (Cr (VI group), the control group (distilled water) and the chromium contaminated well water group (34.91 (Cr (VI) were randomly divided into 8 groups. 349.1698.2, 1396.4 mg 路L potassium dichromate alone and Vit C (100mg/ml) 698.2, 1396.4 mg / L potassium dichromate combined treatment group, 10 rats in each group. The exposure capacity of potassium dichromate and well water group was 25ml kg Vit C was 5ml kg, and then potassium dichromate was exposed to potassium dichromate 0.5 h later. Continuous exposure for 30 days The poisoning performance of mice was observed during the experiment, and the blood and liver tissue were collected after exposure. The contents of blood glucose (Glu) and glutamic alanine aminotransferase (ALT) in serum aspartate aminotransferase (AST),) and glutamic alanine aminotransferase (ALT) were measured by automatic biochemical analyzer, (SOD) activity and malondialdehyde (MDA) content in liver tissue were detected by visible spectrophotometry, and pathomorphological changes of hepatocytes were observed by HE staining. Results the mice in well water group and potassium dichromate group showed obvious poisoning symptoms in the process of exposure. Compared with the control group, the serum AST,ALT activity and liver MDA content of mice exposed to well water group and different concentrations of potassium dichromate alone were higher, while the liver SOD activity, GSH content and serum Glu content were lower, the difference was statistically significant (P < 0.05), but there were no significant changes in serum AST,ALT activity and Glu content, SOD activity and GSH,MDA content in liver of mice pretreated with Vit C. With the increase of potassium dichromate concentration, the activity of serum AST,ALT and the content of MDA in liver of mice increased, while the activity of SOD and the content of GSH in liver decreased. Compared with the group exposed to the same concentration of potassium dichromate alone, the activity of serum AST,ALT and the content of MDA in liver of mice pretreated with VitC were lower, while the contents of serum Glu, SOD activity and GSH in liver were significantly higher than those in group treated with potassium dichromate alone (P < 0.05). Compared with the 34.91mg/L potassium dichromate alone group, the serum AST,ALT activity and liver MDA content in the well water group were higher, while the serum Glu content, liver SOD activity and GSH content were lower, the difference was statistically significant (P < 0.05). Conclusion groundwater in Cr (VI) contaminated area can cause liver injury in mice, and there may be other heavy metal pollution, while Vit C has antagonistic effect on liver injury induced by potassium dichromate.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
本文编号：2504378
[Abstract]:Objective to investigate the effect of groundwater on liver and blood glucose in mice polluted by hexavalent chromium [Cr (VI)] and the intervention effect of vitamin C (VitC). Methods 80 healthy male Kunming mice of 6 weeks old and 8 weeks old were randomly divided into 8 groups: control group (distilled water) group, chromium contaminated well water (12.35mg/L) group (12.35mg/L) and 34.91 (Cr (VI (12.35mg/L) group, respectively, the control group (distilled water), the chromium contaminated well water (12.35mg/L) group and 34.91 (Cr (VI group), the control group (distilled water) and the chromium contaminated well water group (34.91 (Cr (VI) were randomly divided into 8 groups. 349.1698.2, 1396.4 mg 路L potassium dichromate alone and Vit C (100mg/ml) 698.2, 1396.4 mg / L potassium dichromate combined treatment group, 10 rats in each group. The exposure capacity of potassium dichromate and well water group was 25ml kg Vit C was 5ml kg, and then potassium dichromate was exposed to potassium dichromate 0.5 h later. Continuous exposure for 30 days The poisoning performance of mice was observed during the experiment, and the blood and liver tissue were collected after exposure. The contents of blood glucose (Glu) and glutamic alanine aminotransferase (ALT) in serum aspartate aminotransferase (AST),) and glutamic alanine aminotransferase (ALT) were measured by automatic biochemical analyzer, (SOD) activity and malondialdehyde (MDA) content in liver tissue were detected by visible spectrophotometry, and pathomorphological changes of hepatocytes were observed by HE staining. Results the mice in well water group and potassium dichromate group showed obvious poisoning symptoms in the process of exposure. Compared with the control group, the serum AST,ALT activity and liver MDA content of mice exposed to well water group and different concentrations of potassium dichromate alone were higher, while the liver SOD activity, GSH content and serum Glu content were lower, the difference was statistically significant (P < 0.05), but there were no significant changes in serum AST,ALT activity and Glu content, SOD activity and GSH,MDA content in liver of mice pretreated with Vit C. With the increase of potassium dichromate concentration, the activity of serum AST,ALT and the content of MDA in liver of mice increased, while the activity of SOD and the content of GSH in liver decreased. Compared with the group exposed to the same concentration of potassium dichromate alone, the activity of serum AST,ALT and the content of MDA in liver of mice pretreated with VitC were lower, while the contents of serum Glu, SOD activity and GSH in liver were significantly higher than those in group treated with potassium dichromate alone (P < 0.05). Compared with the 34.91mg/L potassium dichromate alone group, the serum AST,ALT activity and liver MDA content in the well water group were higher, while the serum Glu content, liver SOD activity and GSH content were lower, the difference was statistically significant (P < 0.05). Conclusion groundwater in Cr (VI) contaminated area can cause liver injury in mice, and there may be other heavy metal pollution, while Vit C has antagonistic effect on liver injury induced by potassium dichromate.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
【参考文献】
相关期刊论文 前8条
1 考庆君;吴坤;邓晶;谢立;孙昼;黄仁杰;;三价铬和六价铬对大鼠长期慢性毒性的比较[J];癌变.畸变.突变;2007年06期
2 吴兰香;;某化工厂铬污染对工人和居民的健康影响[J];公共卫生与预防医学;2010年04期
3 王晓峰;徐立红;;Cr(Ⅵ)染毒对小鼠肝脏细胞凋亡以及凋亡相关蛋白的影响[J];环境科学学报;2008年03期
4 赵淑华,李景舜,叶琳,王春华,潘维平,石瑞平;口服六价铬对小鼠氧化性损伤及维生素C保护作用的初步研究[J];环境与健康杂志;2000年03期
5 孙明昌;肝病致低血糖昏迷18例[J];江西医药;2004年04期
6 徐延平,刘伟;低血糖40例临床分析[J];中国全科医学;2004年18期
7 史黎薇;铬化合物对健康影响的研究进展[J];卫生研究;2003年04期
8 王取南,孙美芳,魏凌珍,佘素贞,徐德祥;铬致大鼠肾损害与脂质过氧化的实验研究[J];安徽医科大学学报;2000年02期
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