植物甾醇对高脂膳食小鼠骨骼肌蛋白质合成及降解的影响

发布时间：2019-08-10 06:48

【摘要】：目的研究植物甾醇对高脂膳食小鼠骨骼肌中蛋白质合成和降解的调节作用及其可能的机制。方法 32只雄性C57BL/6小鼠随机分为四组:正常日粮组(C)、高脂日粮组(HF)、低剂量植物甾醇组(HF+0.008%PS)和高剂量植物甾醇组(HF+0.016%PS)。实验17 w后运用综合实验动物监测系统检测活动情况、能量摄入和耗氧量,处死后测定腿肌率和肌肉总蛋白含量,Real-time PCR测定肌肉生长及蛋白质代谢相关m RNA的表达。结果高脂日粮导致小鼠体内自由基水平显著升高,肌肉总蛋白含量、腿肌率和夜间活动能力显著下降(P0.05)。植物甾醇能有效清除自由基,显著提高肌肉总蛋白含量和腿肌率,改善小鼠夜间活动能力(P0.05)。与正常组相比,高脂组小鼠骨骼肌中PI3K、Akt、m TOR和Myogenin表达显著下调(P0.05),而Fox O3a、Mu RF1、MAFbx和Myostatin表达显著上调(P0.05),植物甾醇能有效改善高脂饮食造成的上述基因变化(P0.05)。结论植物甾醇能有效改善高脂饮食对小鼠肌肉生长的抑制作用,促进骨骼肌中蛋白质合成并抑制蛋白质降解,从而提高肌肉蛋白质水平,改善小鼠活动能力,促进肌肉生长。
[Abstract]:Objective to study the regulatory effect of plant sterol on protein synthesis and degradation in skeletal muscle of mice fed with high fat diet and its possible mechanism. Methods 32 male C57BL/6 mice were randomly divided into four groups: normal diet group (C), high fat diet group (HF), low dose plant sterol group (HF 0.008%PS) and high dose plant sterol group (HF 0.016%PS). After 17 weeks of experiment, the activity, energy intake and oxygen consumption were detected by comprehensive experimental animal monitoring system, leg muscle rate and muscle total protein content were measured after death, and the expression of m RNA related to muscle growth and protein metabolism was measured by Real-time PCR. Results High fat diet significantly increased free radical level, decreased muscle total protein content, leg muscle rate and nocturnal activity in mice (P 0.05). Plant sterol could effectively eliminate free radicals, significantly increase muscle total protein content and leg muscle rate, and improve nocturnal activity of mice (P 0.05). Compared with the normal group, the expression of PI3K,Akt,m TOR and Myogenin in skeletal muscle of mice in hyperlipidemia group was significantly down-regulated (P 0.05), while the expression of Mu RF1,MAFbx and Myostatin in Fox O3A was significantly up-regulated (P 0.05). Phytosterol could effectively improve the above gene changes caused by high-fat diet (P 0.05). Conclusion Plant sterol can effectively improve the inhibitory effect of high fat diet on muscle growth in mice, promote protein synthesis and inhibit protein degradation in skeletal muscle, thus increase muscle protein level, improve the activity of mice and promote muscle growth.
【作者单位】：江南大学食品学院营养与功能因子研究中心;食品科学与技术国家重点实验室江南大学;
【基金】：国家自然科学基金(No.2012BAD33B05)
【分类号】：R151

【共引文献】