一次静脉注射纳米四氧化三铁对小鼠肝功能的影响

发布时间：2019-08-17 13:21

【摘要】：目的研究纳米四氧化三铁(nano-Fe3O4)对小鼠肝功能的影响。方法 6~8周龄的CD-1(ICR)雄性小鼠81只,随机分为9组,分别将粒径为6.3和54.6 nm的nano-Fe3O4以10 mg/kg体重剂量进行尾静脉注射染毒;染毒后的1、3和7 d处死小鼠,测定小鼠肝脏系数、血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;测定小鼠肝脏谷胱甘肽(GSH)水平,谷胱甘肽过氧化物酶(GSH-Px)与超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量。结果与对照组比较,染毒后3和7 d,小鼠肝脏系数降低,差异有统计学意义(P0.05);血清ALT、AST水平在染毒后1 d显著增高,差异有统计学意义(P0.05),之后逐渐恢复正常水平;肝组织GSH水平在染毒后1 d,显著高于对照组(P0.05);而染毒后1、3和7 d,小鼠肝脏GSH-Px和SOD活性、MDA含量,对照组与各组间差异均无统计学意义(P0.05)。结论单次尾静脉注射两种粒径的nano-Fe3O4,都能引起小鼠肝组织低水平的氧化应激,均未见肝组织明显损伤,两粒径的nanoFe3O4毒性无差异。
[Abstract]:Objective to study the effect of nanometer iron tetroxide (nano-Fe3O4) on liver function in mice. Methods 81 male CD-1 (ICR) mice aged 6 to 8 weeks were randomly divided into 9 groups. Nano-Fe3O4 with particle size of 6.3 and 54.6 nm was injected intravenously with 10 mg/kg body weight, and the mice were killed on the 1st, 3rd and 7th day after exposure, and the liver coefficient and serum alanine transaminase (ALT), aspartate transaminase (AST) level were measured. The levels of glutathione (GSH), the activities of GSH-Px and (SOD), and the content of malondialdehyde (MDA) in the liver of mice were measured. Results compared with the control group, the liver coefficient of mice decreased at 3 and 7 days after exposure, the difference was statistically significant (P 0.05), the serum ALT,AST level increased significantly on the 1st day after exposure, the difference was statistically significant (P 0.05), and then gradually returned to the normal level. The GSH level in liver tissue was significantly higher than that in the control group 1 day after exposure (P 0.05). On the 1st and 7th day after exposure, there was no significant difference in GSH-Px and SOD activity and MDA content between the control group and each group (P 0.05). Conclusion single tail vein injection of two kinds of nano-Fe3O4, can induce low level of oxidative stress in liver tissue of mice, and there is no obvious damage to liver tissue, and there is no difference in the toxicity of nanoFe3O4 between the two particles.
【作者单位】：南华大学公共卫生学院;中国科学院高能物理研究所;中国海洋大学;
【基金】：国家自然科学基金(11275215,11275218)
【分类号】：R114

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