食品中大肠菌群快速检测效果评价

发布时间：2019-08-17 20:25

【摘要】：目的分析复合显色培养基在食品检验中快速测定大肠菌群的作用。方法以大肠菌群标准菌株及其他食源性致病菌标准菌株、85份食品样品为研究对象对象,同时采取国标GB4789.3中对大肠菌群的检测方法以及复合显色培养法进行大肠菌群的检测,比较两者的检测时间准确性等。综合评价该复合显色培养基的效果。结果将标准菌株大肠杆菌、弗氏柠檬酸杆菌、阴沟肠杆菌、阪崎肠杆菌、产气肠杆菌和肺炎克雷伯菌接种在复合显色平板及VRBA平板,均见生长,呈蓝绿色。而福氏志贺菌、副溶血性弧菌和金黄色葡萄球菌非大肠菌群属细菌在复合显色平板上培养18 h后,菌落无生长。检测食品样本的阳性率与国标大肠菌群VRBA平板法差异无统计学意义(P0.05)。结论复合显色培养基可以快速检测食物中大肠菌群并且准确度高,值得在食品检测中使用。
[Abstract]:Objective to analyze the effect of compound color culture medium on rapid determination of coliform bacteria in food inspection. Methods 85 food samples were studied by using standard strains of coliform group and other standard strains of foodborne pathogenic bacteria. At the same time, the detection method of coliform group and compound color culture method in national standard GB4789.3 were used to detect coliform group, and the detection time of coliform group was compared. Accuracy, etc. The effect of the compound color culture medium was evaluated comprehensively. Results the standard strains of Escherichia coli, citric acid bacillus, Enterobacter cloacae, Enterobacter sakazakii, Enterobacter aerogenes and Klebsiella pneumoniae were inoculated on the compound color plate and VRBA plate, and all of them were blue-green. However, Shigella flexneri, Vibrio parahaemolyticus and Staphylococcus aureus non-coliform bacteria were cultured on the compound color plate for 18 hours, and the colony did not grow. There was no significant difference between the positive rate of food samples and the national standard coliform group VRBA plate method (P 0.05). Conclusion the compound color culture medium can quickly detect coliform bacteria in food and has high accuracy, so it is worth using in food detection.
【作者单位】：河北经贸大学生物科学与工程学院;
【分类号】：R155.5

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