Time-dependent gene expression analysis after mouse skeletal

发布时间：2017-10-14 15:39

  本文关键词：小鼠骨骼肌挫伤后基因表达的时间进程（英文）

  更多相关文章： Chemokines Contusion Cytokines Gene Macrophages Satellite cells Skeletal muscle

【摘要】：Background:Though the mechanisms of skeletal muscle regeneration are deeply understood,those involved in muscle contusion,one of the most common muscle injuries in sports medicine clinics,are not.The objective of this study is to explore the mechanisms involved in muscle regeneration after contusion injury.Methods:In this study,a total of 72 mice were used.Eight of them were randomly chosen for the control group,while the rest were subjected to muscle contusion.Subsequently,their gastrocnemius muscles were harvested at different time points.The changes in muscle morphology were assessed by hematoxylin and eosin(HE) stain.In addition,the gene expression was analyzed by real-time polymerase chain reaction.Results:The data showed that the expression of many genes,i.e.,specific markers of immune cells and satellite cells,regulatory factors for muscle regeneration,cytokines,and chemokines,increased in the early stages of recovery,especially in the first 3 days.Furthermore,there were strict rules in the expression of these genes.However,almost all the genes returned to normal at 14 days post-injury.Conclusion:The sequence of immune cells invaded after muscle contusion was neutrophils,M1 macrophages and M2 macrophages.Some CC(CCL2,CCL3,and CCL4) and CXC(CXCL10) chemokines may be involved in the chemotaxis of these immune cells.HGF may be the primary factor to activate the satellite cells after muscle contusion.Moreover,2 weeks are needed to recover when acute contusion happens as used in this study.
【作者单位】： School
【关键词】： Chemokines Contusion Cytokines Gene Macrophages Satellite cells Skeletal muscle
【基金】：supported by the grants from National Natural Science Foundation of China (No.31271273,No.31300975) the Doctoral Fund of Ministry of Education of China (No.20133156120004) the Key Lab of Exercise and Health Sciences of Ministry of Education (Shanghai University of Sport)
【分类号】：G804.2
【正文快照】： 1.IntroductionMuscle injuries are common musculoskeletal problems.Themechanisms of skeletal muscle recovery are becoming clearerrecently.The healing process of injured skeletal muscle consistsof three distinct phases:degeneration and inflammation,regen-e

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