移植时间和检测方法与allo-HSCT受者的DNA嵌合率及法医学意义

发布时间：2018-01-13 11:57

本文关键词：移植时间和检测方法与allo-HSCT受者的DNA嵌合率及法医学意义　出处：《中国法医学杂志》2016年05期 　论文类型：期刊论文

【摘要】：影响allo-HSCT受者DNA嵌合率的因素有很多:如受者的疾病类型、移植前预处理方案、移植时病情的严重程度、造血干细胞来源、局部的GVHD、HLA是否相合等。本文主要讨论与法医学关系最为密切的2个影响因素,即移植后时间和检测方法的灵敏度。法医物证学工作者在检案实践中,对allo-HSCT受者进行亲权鉴定或个体识别时,需注意移植后时间和检测方法的灵敏度对嵌合率的影响。女性受者接受男性供者HSCT的情况下,受者毛囊中可以检出供者Y染色体DNA,检案实践中来源于犯罪现场的生物检材不能仅仅检测Y染色体特异遗传标记,以免导致错误判断。
[Abstract]:There are many factors that influence the chimeric rate of DNA in allo-HSCT recipients, such as the type of disease, preconditioning, the severity of the disease during transplantation, and the origin of hematopoietic stem cells. This paper mainly discusses the two influencing factors which are most closely related to forensic medicine. That is, the time after transplantation and the sensitivity of detection methods. In the practice of forensic forensics, the allo-HSCT recipients were identified by paternity or individual identification. Attention should be paid to the effect of time after transplantation and sensitivity of detection methods on chimerism rate. When female recipients received male donor HSCT, donor Y chromosome DNA could be detected in the hair follicles of recipients. In practice, biological samples from crime scene can not only detect Y chromosome specific genetic markers, so as to avoid misjudgment.
【作者单位】：山东省高校证据鉴识重点实验室;山东政法学院司法鉴定中心;舟山市公安局刑事科学技术研究所;
【基金】：山东政法学院科研计划项目(2014F14B) 山东省自然科学基金资助项目(2R2014HQ018)
【分类号】：D919
【正文快照】： 异基因造血干细胞移植(allo-HSCT)已广泛用于恶性血液病、非恶性造血系统疾病、遗传性疾病及免疫缺陷等疾病的治疗。随着移植技术的不断进步,allo-HSCT的无病生存率和生存时间也逐渐增加,进行移植的病例数逐年上升。造血干细胞(HSC)是具有多向分化潜能的造血前体细胞,供者的HS

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